DBT Antikörper (AA 46-462)

Produktnummer ABIN7985278

Der Kaninchen Polyklonal anti-DBT Antikörper wird verwendet zum Nachweis von DBT in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA, FACS, IF, ICC und IP.

Kurzübersicht für DBT Antikörper (AA 46-462) (ABIN7985278)

Target: DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DBT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Güteklasse: Carrier-free

Produktdetails anti-DBT Antikörper

Bindungsspezifität: AA 46-462

Verwendungszweck: Anti-DBT Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Homologie: Human DBT shares 89.7% amino acid (aa) sequence identity with mouse DBT.

Produktmerkmale: Anti-DBT Antibody. Tested in WB, ICC/IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human DBT recombinant protein (Position: K46-R462). Human DBT shares 89.7% amino acid (aa) sequence identity with mouse DBT.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Immunoprecipitation, 2-4 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu DBT

Target: DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))

Andere Bezeichnung: DBT

Hintergrund:

Background: The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Gene Full Name: dihydrolipoamide branched chain transacylase E2

Molekulargewicht: 53 kDa

Gen-ID: 1629

UniProt: P11182