Dihydrofolate Reductase Antikörper (AA 2-187) (Biotin)

Produktnummer ABIN7985257

Der Maus Monoklonal anti-Dihydrofolate Reductase Antikörper wird verwendet zum Nachweis von Dihydrofolate Reductase in Proben von Human und Ratte. Er wurde validiert für WB, IHC und ELISA.

Kurzübersicht für Dihydrofolate Reductase Antikörper (AA 2-187) (Biotin) (ABIN7985257)

Target: Dihydrofolate Reductase (DHFR)

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Dihydrofolate Reductase Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Klon: 3C8

Produktdetails anti-Dihydrofolate Reductase Antikörper (Biotin)

Bindungsspezifität: AA 2-187

Verwendungszweck: Anti-DHFR Antibody (monoclonal, 3C8) Biotin Conjugated

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Homologie: Human DHFRshares90%aminoacid (aa)sequenceidentitywith both mouseandrat DHFR.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human DHFR recombinant protein (Position: V2-D187). Human DHFRshares90%aminoacid (aa)sequenceidentitywith both mouseandrat DHFR.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu Dihydrofolate Reductase

Target: Dihydrofolate Reductase (DHFR)

Andere Bezeichnung: DHFR

Hintergrund:

Background: SHC, also known as SHC1 (SHC-transforming protein 1) or SHCA, is a protein that in humans is encoded by the SHC1 gene. SCOP classifies the 3D structure as belonging to the SH2 domain family. This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene.

Gene Full Name: dihydrofolate reductase

Gen-ID: 1719

UniProt: P00374

Pathways: Mitotic G1-G1/S Phases