DBF4 Antikörper (AA 295-665)

Produktnummer ABIN7983808

Der Kaninchen Polyklonal anti-DBF4 Antikörper wird verwendet zum Nachweis von DBF4 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA, IF, FACS und ICC.

Kurzübersicht für DBF4 Antikörper (AA 295-665) (ABIN7983808)

Target: DBF4 (DBF4 Homolog (DBF4))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DBF4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Güteklasse: Carrier-free

Produktdetails anti-DBF4 Antikörper

Bindungsspezifität: AA 295-665

Verwendungszweck: Anti-DBF4 Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-DBF4 Antibody. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human DBF4 recombinant protein (Position:Y295-S665).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu DBF4

Target: DBF4 (DBF4 Homolog (DBF4))

Andere Bezeichnung: DBF4

Hintergrund:

Background: Using both gain- and loss-of-function experiments, it was found that Xenopus Dbf4 inhibited canonical Wnt signaling. Depletion of endogenous Dbf4 did not disturb gastrulation movements or early organizer genes, but resulted in embryos with morphologically defective heart and eyes and suppressed cardiac markers. The function of Dbf4 in heart development appeared to be independent of its role in the cell cycle. Dbf4 interacted physically and functionally with Frodo, a context-dependent regulator of Wnt signaling.

Gene Full Name: DBF4-CDC7 kinase regulatory subunit

Molekulargewicht: 80 kDa

Gen-ID: 10926

UniProt: Q9UBU7

Pathways: Mitotic G1-G1/S Phases, DNA Replication