CD68 Antikörper (Middle Region)

Produktnummer ABIN7977284

Der Kaninchen Polyklonal anti-CD68 Antikörper (ABIN7977284) detektiert spezifisch CD68 in IHC, FACS, WB und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Maus und Ratte.

Kurzübersicht für CD68 Antikörper (Middle Region) (ABIN7977284)

Target: CD68 (CD68 Molecule (CD68))

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD68 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF)

Güteklasse: Carrier-free

Produktdetails anti-CD68 Antikörper

Bindungsspezifität: Middle Region

Verwendungszweck: Anti-Macrosialin CD68 Antibody

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Homologie: different from the related rat sequence by one amino acid.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of mouse CD68, different from the related rat sequence by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat Immunofluorescence, 5 μg/mL, Mouse Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu CD68

Target: CD68 (CD68 Molecule (CD68))

Andere Bezeichnung: CD68

Hintergrund:

Background: CD68 is a heavily glycosylated glycoprotein that is highly expressed in macrophages and other mononuclear phagocytes. Macrosialin, if not glycosylated, has the main sequence that consists of 354 amino acids with an expected molecular weight of 37.4 kD. CD68, which can be located in the cytoplasmic granules of a variety of blood cells and myocytes, can be detected via immunohistochemistry. It's particularly effective as a marker for monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts, all of which belong to the macrophage lineage. CD68 is expressed in tissue macrophages and to a lesser extent in dendritic cells. CD68 is involved in tissue macrophage phagocytic activities, as well as intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It works by binding to tissue and organ-specific lectins or selectins, enabling macrophage subsets to be ed to specific locations. Macrophages may be able to crawl over selectin-bearing substrates or other cells if CD68 is rapidly recirculated from endosomes and lysosomes to the plasma membrane.

Gene Full Name: CD68 antigen

Sequence Similarities: Belongs to the LAMP family.

Molekulargewicht: 90-100 kDa

Gen-ID: 12514

UniProt: P31996