AQP2 Antikörper (AA 102-271) (PE)

Produktnummer ABIN7969672

Der Kaninchen Polyklonal anti-AQP2 Antikörper wird verwendet zum Nachweis von AQP2 in Proben von Ratte und Maus. Er wurde validiert für FACS.

Kurzübersicht für AQP2 Antikörper (AA 102-271) (PE) (ABIN7969672)

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Reaktivität: Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AQP2 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Produktdetails anti-AQP2 Antikörper (PE)

Bindungsspezifität: AA 102-271

Verwendungszweck: Anti-Aqp2 Antibody PE Conjugated

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Homologie: Mouse Aqp2 shares 87.6%,98.2% amino acid (aa) sequence identity with human,rat Aqp2,respectively.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Aqp2 recombinant protein (Position: A102-A271). Mouse Aqp2 shares 87.6% and 98.2% amino acid (aa) sequence identity with human and rat Aqp2, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Flow Cytometry, Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Haltbarkeit: 12 months

Details zu AQP2

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Andere Bezeichnung: Aqp2

Hintergrund:

Background: AQP2 (Aquaporin 2), also called AQUAPORIN-CD, is found in the apical cell membranes of the kidney's collecting duct principal cells and in intracellular vesicles located throughout the cell. The AQP2 gene is mapped to chromosome 12q13, very close to the site of major intrinsic protein by situ hybridization. The investigators suggested that a defect in the AQP2 gene is the basis of the autosomal form of nephrogenic diabetes insipidus. The functional expression and the limited localization suggested that AQP2 is the vasopressin-regulated water channel. Using rat kidney slices and porcine kidney cells stably expressing rat Aqp2, AQP2 trafficking can be stimulated by cAMP-independent pathways that utilize nitric oxide (NO). The NO donors sodium nitroprusside (SNP) and NONOate and the NO synthase substrate L-arginine mimicked the effect of vasopressin (VP), stimulating relocation of Aqp2 from cytoplasmic vesicles to the apical plasma membrane. SNP increased intracellular cGMP rather than cAMP, and exogenous cGMP stimulated AQP2 membrane insertion. Atrial natriuretic factor, which signals via cGMP, also stimulated AQP2 translocation. AQP2 expression in kidney connecting tubules is sufficient for survival and that AQP2 expression in collecting ducts is required to regulate body water balance. The S256L substitution in the cytoplasmic tail of the Aqp2 protein prevented phosphorylation at S256 and the subsequent accumulation of Aqp2 on the apical membrane of the collecting duct principal cells.

Gene Full Name: aquaporin 2

Gen-ID: 11827

UniProt: P56402

Pathways: Response to Water Deprivation