SUZ12 Antikörper (C-Term)

Produktnummer ABIN7938516

Der Kaninchen Polyklonal anti-SUZ12 Antikörper (ABIN7938516) detektiert spezifisch SUZ12 in WB, IF, ChIP, ChIP-seq und CUT&RUN. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für SUZ12 Antikörper (C-Term) (ABIN7938516)

Target: SUZ12 (SUZ12 Polycomb Repressive Complex 2 Subunit (SUZ12))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SUZ12 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)

Produktdetails anti-SUZ12 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Suz12 antibody (pAb)

Aufreinigung: Affinity Purified

Immunogen: This Suz12 antibody was raised against a peptide corresponding to the C-terminus of human Suz12.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ChIP: 10 µL per ChIP ChIP-Seq: 10 µL each WB*: 1:250 - 1:1,000 dilution CUT&RUN: 1 µL per 50 µL reaction *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. An additional band at approximately 20 kDa is also detected by this antibody in HeLa and HEK lysates, but not in mouse CSML0 cell lysate. For Suz12, we also offer AbFlex Suz12 Recombinant Antibody (rAb). For details, see Catalog No. 91163.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, 30 % glycerol, 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu SUZ12

Target: SUZ12 (SUZ12 Polycomb Repressive Complex 2 Subunit (SUZ12))

Andere Bezeichnung: Suz12

Hintergrund:

Background_Plain: Suz12 (Suppressor-of-Zeste 12 protein, also designated as ChET9, JJAZ1 protein) is a component of the Polycomb group complexes 2, 3 and 4 (PRC2, PRC3 and PRC4), which also contain the histone methyltransferase EZH2, EED (Embryonic Ectoderm Development protein) protein and the histone-binding proteins RbAP46 and RbAP48. PRC2 is responsible for di- and trimethylation of histone 3 at lysine 27 (Histone H3K27me2 and Histone H3K27me3), which leads to transcriptional repression of the target genes. Suz12 is a nuclear protein belonging to the VEFS family. Suz12 contains a zinc-finger binding motif and a VEFS box, which is required for the interaction between Suz12 and EZH2.

Aliases: CHET9, JJAZ1

Molekulargewicht: 95 kDa

NCBI Accession: NP_056170