SUPT5H Antikörper (C-Term)

Produktnummer ABIN7938512

Der Ratte Monoklonal anti-SUPT5H Antikörper wird verwendet zum Nachweis von SUPT5H in Proben von Human. Er wurde validiert für WB, IP und ChIP-seq.

Kurzübersicht für SUPT5H Antikörper (C-Term) (ABIN7938512)

Target: SUPT5H (Transcription Elongation Factor SPT5 (SUPT5H))

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser SUPT5H Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq)

Klon: 6F1

Produktdetails anti-SUPT5H Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Supt5H/SPT5 antibody (mAb) (Clone 6F1)

Aufreinigung: Protein G Chromatography

Immunogen: This antibody was raised against a synthetic peptide derived from the human SPT5 C-terminal region.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ChIP-Seq: 4 μg per ChIP WB*: 0.5 - 2 μg/mL IP: 5 μg/mL *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91331.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu SUPT5H

Target: SUPT5H (Transcription Elongation Factor SPT5 (SUPT5H))

Andere Bezeichnung: Supt5H/SPT5

Hintergrund:

Background_Plain: SUPT5H (SPT5 Homolog, DSIF Elongation Factor Subunit) is a transcription elongation factor which regulates mRNA processing and transcription elongation by RNA polymerase II. SUPT5H positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. SUPT5H also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Diseases associated with SUPT5H include HIV-1. Among its related pathways are mRNA capping and HIV life cycle.

Aliases: SPT5 Homolog, DSIF Elongation Factor Subunit, DSIF Large Subunit, Transcription Elongation Factor SPT5

Molekulargewicht: 140 kDa

NCBI Accession: NP_001104490