POLR2F Antikörper (meLys7)

Produktnummer ABIN7938446

Dieser Ratte Monoklonal Antikörper detektiert spezifisch POLR2F in WB, ChIP, IP und ChIP-seq. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für POLR2F Antikörper (meLys7) (ABIN7938446)

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser POLR2F Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq)

Klon: 1F5

Produktdetails anti-POLR2F Antikörper

Bindungsspezifität: meLys7

Verwendungszweck: RNA pol II CTD monomethyl Lys7 antibody (mAb) (Clone 1F5)

Aufreinigung: Protein G Chromatography

Immunogen: This antibody was raised against a peptide containing dimethyl-lysine 7 of human RNA Pol II CTD.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: ChIP-Seq: 10 μg per ChIP IP: 5 μg per IP WB*: 0.5 - 2 μg/mL *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.05 % BSA and 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu POLR2F

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Andere Bezeichnung: RNA pol II

Hintergrund:

Background_Plain: Genome-wide profiling revealed high levels of K7me1/2 marks at the transcriptional start site of genes for sense and antisense transcribing RNAPII. The new K7 modifications further expand the mammalian CTD code to allow regulation of differential gene expression. In analogy, it appears likely that the various modifications of K7 residues identified in this analysis may similarly contribute to fine tuning of gene expressing by RNAPII, and that these modifications are placed in K7 residues of CTD dependent on cell signaling and epigenetic programming of cells.

Aliases: RNA Polymerase II Carboxy Terminal Domain

Molekulargewicht: 240 kDa

NCBI Accession: NP_000928

Pathways: Regulatorische RNA Pathways