PCIF1 Antikörper (C-Term)

Produktnummer ABIN7938398

Der Kaninchen Polyklonal anti-PCIF1 Antikörper wird verwendet zum Nachweis von PCIF1 in Proben von Human. Er wurde validiert für WB und IP.

Kurzübersicht für PCIF1 Antikörper (C-Term) (ABIN7938398)

Target: PCIF1 (PDX1 C-terminal Inhibiting Factor 1 (PCIF1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PCIF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-PCIF1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: PCIF1/CAPAM antibody (pAb)

Aufreinigung: Protein A Chromatography

Immunogen: This antibody was raised against a peptide within the C-terminal region of human PCIF1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IP: 10 µL per IP WB*: 0.5-2 μg/mL *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. For optimal IP results, the addition of 0.05 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified IgG in PBS, 30 % glycerol, and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu PCIF1

Target: PCIF1 (PDX1 C-terminal Inhibiting Factor 1 (PCIF1))

Andere Bezeichnung: PCIF1

Hintergrund:

Background_Plain: The abundant modification N6-methyladenosine (m6A), in eukarytic mRNAs, has been identified as a major epitranscriptome mark that plays critical roles in RNA metabolism and function. In addition to the internal m6A, N6, 2-O dimethyladenosine (m6Am) is present at the transcription start site of capped mRNAs in vertebrates. The abundance of this modification in the transcriptome has been quantified and identified as the writer protein, cap-specific adenosine methyltransferase (CAPAM/PCIF1), is needed for this modification. CAPAM/PCIF1 is identified as the Methyltransferase responsible for methylating 2-O-methyladenosine (Am) at its N6 position to generate m6Am. m6Am is involved in regulating translation of capped mRNAs. Depleting CAPAM/PCIF1 does not affect mRNA translation but is associated with reduced stability of a subset of m6Am annotated mRNAs.

Aliases: Transcription factor PCF1, Phosphorylated CTD Interacting Factor 1 (PCIF1)

Molekulargewicht: 80 kDa

NCBI Accession: NP_071387