Histone H4 Antikörper (H4K16ac)

Produktnummer ABIN7938255

Der Kaninchen Polyklonal anti-Histone H4 Antikörper (ABIN7938255) detektiert spezifisch Histone H4 in WB, IF, ChIP, DB, ChIP-seq und CUT&Tag. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Saccharomyces cerevisiae und Drosophila melanogaster.

Kurzübersicht für Histone H4 Antikörper (H4K16ac) (ABIN7938255)

Target: Histone H4 (HISTH4)

Reaktivität: Human, Maus, Saccharomyces cerevisiae, Drosophila melanogaster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone H4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Tagmentation (CUT&Tag)

Produktdetails anti-Histone H4 Antikörper

Bindungsspezifität: H4K16ac

Verwendungszweck: Histone H4K16ac antibody (pAb)

Aufreinigung: Unpurified

Immunogen: This Histone H4 acetyl Lys16 antibody was raised against a peptide including acetyl-lysine 16 of histone H4.

Anwendungsinformationen

Applikationshinweise: ChIP: 10 µL per ChIP ChIP-Seq: 10 µL each WB: 1:1,000 - 1:5,000 dilution CUT&Tag: 1-2 µL per 50 µL reaction ChIP-Seq validation was performed by Active Motif's Epigenetics Services, the complete data set is available in the UCSC Genome Browser by clicking here. For optimal results in Western blotting, primary antibody incubations should be performed overnight at 4 °C. Individual optimization may be required.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu Histone H4

Target: Histone H4 (HISTH4)

Andere Bezeichnung: Histone H4

Hintergrund: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. NoRC is a SMARCA5 (SNF2h)-containing chromatin remodeling complex. The bromodomain of TIP5, the large subunit of NoRC, interacts with acetylated Histone H4 Lys16, (H4K16ac) and cooperates with an adjacent PHD finger to recruit histone deacetylases and DNA methyltransferases to rDNA, leading to the silencing of rDNA.

Molekulargewicht: 8 kDa

NCBI Accession: NP_778224