BRD9 Antikörper (C-Term)

Produktnummer ABIN7938072

Der Kaninchen Polyklonal anti-BRD9 Antikörper (ABIN7938072) detektiert spezifisch BRD9 in WB und IP. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für BRD9 Antikörper (C-Term) (ABIN7938072)

Target: BRD9 (Bromodomain Containing 9 (BRD9))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BRD9 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-BRD9 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: BRD9 antibody (pAb)

Aufreinigung: Affinity Purified

Immunogen: This antibody was raised against a peptide within the C-terminal region of human BRD9 (bromodomain containing 9).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IP: 5 µL per IP WB*: 1:250 - 1:1,000 dilution *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. For optimal IP results, the addition of 0.05 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu BRD9

Target: BRD9 (Bromodomain Containing 9 (BRD9))

Andere Bezeichnung: BRD9

Hintergrund:

Background_Plain: BRD9 (bromodomain containing 9) belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as 'readers' of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6) that function in the regulation of transcriptional activation and chromatin remodeling. There are five isoforms of BRD9 that are produced by alternative splicing.

Aliases: PRO9856, LAVS3040

Molekulargewicht: 85 kDa

NCBI Accession: NP_076413