5-Carboxylcytosine Antikörper

Produktnummer ABIN7938024

Der Kaninchen Polyklonal anti-5-Carboxylcytosine Antikörper (ABIN7938024) detektiert spezifisch 5-Carboxylcytosine in DB, IF, ICC, IHC und WB.

Kurzübersicht für 5-Carboxylcytosine Antikörper (ABIN7938024)

Target: 5-Carboxylcytosine

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Dot Blot (DB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)

Produktdetails

Verwendungszweck: 5-Carboxylcytosine (5-caC) antibody (pAb)

Aufreinigung: Unpurified

Immunogen: This 5-Carboxylcytosine antibody was raised against 5-carboxylcytidine conjugated to KLH and recognizes 5-carboxylcytosine.

Anwendungsinformationen

Applikationshinweise: DB: 1:5,000 dilution

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Antigendetails

Target: 5-Carboxylcytosine

Substanzklasse: Chemical

Hintergrund: DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC into 5-formylcytosine and 5-carboxylcytosine. An increase in levels of 5-formylcytosine and 5-carboxylcytosine are detected in the mouse male pronucleus following fertilization, which is gradually diluted by DNA replication.