SATB1 Antikörper (pSer47)

Produktnummer ABIN745613

Es gibt 1 Publikation für dieses Produkt. Der Kaninchen Polyklonal anti-SATB1 Antikörper wird verwendet zum Nachweis von SATB1 in Proben von Human und Maus. Er wurde validiert für ELISA, FACS, IHC (p), IF (cc), IF (p) und IHC (fro).

Kurzübersicht für SATB1 Antikörper (pSer47) (ABIN745613)

Target: SATB1 (SATB Homeobox 1 (SATB1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SATB1 Antikörper ist unkonjugiert

Applikation: ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-SATB1 Antikörper

Bindungsspezifität: pSer47

Kreuzreaktivität: Human, Maus

Homologie: Dog,Cow,Pig,Chicken,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human SATB1 around the phosphorylation site of Ser47

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-SATB1 Antikörper (ABIN745613)

Han, Xia, Qin, Han, Wu: "Phosphorylated SATB1 is associated with the progression and prognosis of glioma." in: Cell death & disease, Vol. 4, pp. e901, (2013) (PubMed).

Details zu SATB1

Target: SATB1 (SATB Homeobox 1 (SATB1))

Andere Bezeichnung: SATB1

Hintergrund:

Synonyms: DNA-binding protein SATB1, Special AT-rich sequence-binding protein 1, SATB1

Background: Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma (By similarity). Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes (e.g. PML at the MHC-I locus) and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis. Interacts with the unique region (UR) of cytomegalovirus (CMV). Alu-like motifs and SATB1-binding sites provide a unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL2 and IL2RA (interleukin) in T-cells by binding to the same domain than HDAC1. Delineates specific epigenetic modifications at target gene loci, directly up-regulating metastasis-associated genes while down-regulating tumor-suppressor genes. Reprograms chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis.

Gen-ID: 6304

UniProt: Q01826

Pathways: Caspase Kaskade in der Apoptose, Activated T Cell Proliferation