Syntaxin 4 Antikörper (Intracellular, N-Term)

Produktnummer ABIN7885560

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Syntaxin 4 in WB, IP und IHC. Es zeigt Reaktivität gegenüber Proben von Ratte und Maus.

Kurzübersicht für Syntaxin 4 Antikörper (Intracellular, N-Term) (ABIN7885560)

Target: Syntaxin 4 (STX4)

Reaktivität: Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Syntaxin 4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Güteklasse: Carrier-free

Produktdetails anti-Syntaxin 4 Antikörper

Bindungsspezifität: AA 2-23, Intracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to Syntaxin 4

Homologie: Mouse - identical, human - 17,22 amino acid residues identical

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: RDRTHELRQGDNISDDEDEVRV(C), corresponding to residues 2-23 of rat syntaxin 4

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:100

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7237347)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW)

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu Syntaxin 4

Target: Syntaxin 4 (STX4)

Andere Bezeichnung: STX4

Hintergrund:

Synonyms: STX4, Renal carcinoma antigen NY-REN-31, P35-2

Description: Syntaxin 4 is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein superfamily. The family includes 36 members in humans and is characterized by the SNARE motif, an evolutionarily conserved stretch of 60-70 amino acids that are arranged in heptad repeats1,2.SNARE proteins are involved in exocytosis and intracellular vesicle trafficking and are essential for cell growth, hormone secretion and neurotransmission, processes that require rapid, targeted, and regulated membrane fusion1,2.SNAREs can be roughly divided into vesicular (v-SNAREs) and target (t-SNAREs) based on their distribution on the transport vesicle or target membrane respectively. Thus, assembly of cognate v-/t-SNAREs between two opposing membranes generates trans-SNARE complexes, which bring the lipid bilayers in close proximity and drive membrane fusion.Syntaxin 4, like most SNAREs, is a type IV membrane protein with a relatively large N-terminus containing the SNARE motif located in the cytoplasmic side and a transmembrane domain located close to the C-terminus that functions as an anchor1,2.Syntaxin 4 functions as a plasma membrane protein t-SNARE and has been shown to have an important role in pancreatic b-cell insulin secretion and in insulin-stimulated glucose uptake in skeletal muscle and adipose tissue by means of translocation of the glucose transporter GLUT4 from an intracellular compartment to the plasma membrane3,4.

Gen-ID: 81803

UniProt: Q08850

Pathways: Synaptic Vesicle Exocytosis