RYR3 Antikörper (Intracellular, N-Term)

Produktnummer ABIN7885376

Der Kaninchen Polyklonal anti-RYR3 Antikörper (ABIN7885376) detektiert spezifisch RYR3 in IHC, IF, WB und IC. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für RYR3 Antikörper (Intracellular, N-Term) (ABIN7885376)

Target: RYR3 (Ryanodine Receptor 3 (RYR3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RYR3 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blotting (WB), Immunochromatography (IC)

Güteklasse: Carrier-free

Produktdetails anti-RYR3 Antikörper

Bindungsspezifität: AA 935-949, Intracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to Ryanodine Receptor 3

Homologie: Rat,mouse - identical

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: CHIAHVNPAAEEDLK, corresponding to amino acid residues 935-949 of human RyR3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:100

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7236746)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW)

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu RYR3

Target: RYR3 (Ryanodine Receptor 3 (RYR3))

Andere Bezeichnung: RyR3

Hintergrund:

Synonyms: RyR3, Brain-type ryanodine receptor

Description: It is well established that cytosolic calcium (Ca2+) acts as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The primary intracellular Ca2+ storage/release organelle in most cells is the endoplasmic reticulum (ER) or the sarcoplasmic reticulum (SR) in striated muscle cells.The ER and SR contain two Ca2+ release channel families, the inositol trisphosphate receptors (IP3Rs) and the ryanodine receptors (RyRs).3The ryanodine receptor family consists of three different isoforms: the skeletal muscle isoform, ryanodine receptor type 1 (RyR1), the cardiac muscle isoform, ryanodine receptor type 2 (RyR2) and the brain isoform, ryanodine receptor type 3 (RyR3).3The ryanodine receptors are homotetrameric proteins, which upon assembly create a Ca2+ conducting channel. They play a key role in the mechanism of excitation-contraction coupling in striated muscle. Binding of ryanodine (a poisonous alkaloid found in the South American plant Ryania speciosa) to the ryanodine receptor causes two major changes in the channel: a reduction in single-channel conductance and a marked increase in the open state probability, leading to an overall increase/decrease in the Ca2+ release capability of the channel.RyR3 is the most widely expressed RyR channel which is predominantly expressed in smooth muscle tissues and certain regions of the brain.

Gen-ID: 6263

UniProt: Q15413

Pathways: Myometrial Relaxation and Contraction