Plexin A3 Antikörper (Extracellular, N-Term)

Produktnummer ABIN7885234

Der Kaninchen Polyklonal anti-Plexin A3 Antikörper wird verwendet zum Nachweis von Plexin A3 in Proben von Human, Maus und Ratte. Er wurde validiert für IHC, WB, FACS, IF und LCI.

Kurzübersicht für Plexin A3 Antikörper (Extracellular, N-Term) (ABIN7885234)

Target: Plexin A3 (PLXNA3)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Plexin A3 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Live Cell Imaging (LCI)

Güteklasse: Carrier-free

Produktdetails anti-Plexin A3 Antikörper

Bindungsspezifität: AA 440-453, Extracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to Plexin-A3

Homologie: Rat - identical, human - 12,14 amino acid residues identical

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)KVRVDGSQDAQLYE, corresponding to amino acid residues 440-453 of mouse PLXNA3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7236268)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW)

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu Plexin A3

Target: Plexin A3 (PLXNA3)

Andere Bezeichnung: PLXNA3

Hintergrund:

Synonyms: PLXNA3, PLXN3, Semaphorin receptor SEX

Description: Plexins are type 1 transmembrane (TM) receptors acting as key regulatory proteins in a wide variety of developmental, regenerative and pathological processes. They are involved in guidance and motility of vascular, lymphatic vessel, and neuron growth, and in cancer metastasis.Plexin family includes 9 proteins: Plexin-A1-4, -B1-3, -C1 and -D1. Class A Plexins interact with the secreted class 3 Semaphorins, and require Neuropilins (nrps) as co-receptors.Plexin protein signaling results in cytosolic GTPase-activating protein activity including direct interactions with Rho GTPases and transient/catalytic interactions with Ras GTPases1,2.Plexin structure consists of an extracellular semaphorin domain, three Plexin-Semaphorin-Integrin (PSI) domains, and three immunoglobulin, Plexin, and transcription factor (IPT) domains. In addition, a single-spanning transmembrane domain, and a cytosolic region (CYTO) homologous with Ras GTPase-activating proteins (GAPs)2. The transmembrane region of Plexin-A3 is characterized by poly-glycine usually used for close helix-helix packing and aromatic residues that distort helix conformation and association. Plexin-A family members only have 22 residues spanning the membrane1.Mutations in Plexins have been reported in melanoma, lung, breast, pancreatic, and prostate cancers, strongly suggesting that their signaling may play a role in cancer development. Plexins are widely expressed in the nervous system and in the developing cerebral cortex2.

Gen-ID: 18846

UniProt: P70208

Pathways: Regulation of Cell Size