PANX1 Antikörper (Intracellular, N-Term)

Produktnummer ABIN7885220

Der Kaninchen Polyklonal anti-PANX1 Antikörper (ABIN7885220) detektiert spezifisch PANX1 in WB, IHC und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte und Maus.

Kurzübersicht für PANX1 Antikörper (Intracellular, N-Term) (ABIN7885220)

Target: PANX1 (Pannexin 1 (PANX1))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PANX1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Güteklasse: Carrier-free, KO Validated

Produktdetails anti-PANX1 Antikörper

Bindungsspezifität: AA 18-31, Intracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to Pannexin 1

Homologie: Rat,mouse - identical

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)KEPTEPKFKGLRLE, corresponding to amino acid residues 18- 31 of human PANX1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7236221)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW)

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu PANX1

Target: PANX1 (Pannexin 1 (PANX1))

Andere Bezeichnung: PANX1

Hintergrund:

Synonyms: PANX1, PX1, MRS1, UNQ2529

Description: Gap junctions are usually found in clusters and enable intercellular communication by allowing the passage of small molecules between cells1. They play important roles in different biological processes. These include differentiation, cell cycle synchronization, cellular development, neuronal activity and the immune response2-4.Proteins involved in gap junction formation are composed of four transmembrane domains, two extracellular loops and one intracellular loop and intracellular N- and C-termini5,6. Several consensus cysteine residues are in the extracellular loop and are essential and necessary for intercellular docking of gap junction hemichannels in the opposing cell membrane5-7.Pannexins (Pannexin 1, Pannexin 2 and Pannexin 3) belong to the superfamily of gap junction proteins. Pannexin 1 (PANX1) is ubiquitously expressed, Pannexin 2 (PANX2) is specifically expressed in the human brain and widespread in rodents and Pannexin 3 (PANX3) is also detected in the brain8.The gating properties of Pannexins were studied in Xenopus oocytes and results demonstrate that only PANX1 is able to form homomeric hemichannels, and is also able to form heteromeric hemichannels with PANX2 but not with PANX39,10. Not surprising, PANX1 gating properties depend whether it forms homomeric or heteromeric hemichannels.A number of different stimuli are known to open these channels and include mechanical stress, extracellular ATP, increases in intracellular Ca2+ and inflammation9.Possible roles for Pannexins include paracrine signaling in vascular endothelial cells and taste cell signaling9. Pannexin-1 was shown to interact with NMDA receptors in epilepsy, ischemia, pain and other pathologies10.

Gen-ID: 24145

UniProt: Q96RD7