NPTN Antikörper (Extracellular, N-Term)

Produktnummer ABIN7885193

Der Kaninchen Polyklonal anti-NPTN Antikörper (ABIN7885193) detektiert spezifisch NPTN in WB, IHC, IF, FACS und LCI. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte und Maus.

Kurzübersicht für NPTN Antikörper (Extracellular, N-Term) (ABIN7885193)

Target: NPTN (Neuroplastin (NPTN))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NPTN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Güteklasse: Carrier-free

Produktdetails anti-NPTN Antikörper

Bindungsspezifität: AA 194-210, Extracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to Neuroplastin

Homologie: Mouse,human - identical

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)RKNASNMEYRINKPRAE, corresponding to amino acid residues 194-210 of rat NPTN

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:400

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:120

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7236121)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW)

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu NPTN

Target: NPTN (Neuroplastin (NPTN))

Andere Bezeichnung: NPTN

Hintergrund:

Synonyms: NPTN, Glycoprotein 55/65, GP55, GP65, Np55, Np65, Stromal cell-derived receptor 1, SDR1, SDFR1

Description: The neuroplastins Np55 and Np65 comprise a superfamily of neuronal and synapse enriched immunoglobulin molecules. They play an important role in a number of vital synaptic and neuronal activities including cell adhesion in the case of Np65. Np65 and Np55 are identical in structure except for the N-terminal Np65-specific Ig1 domain. They also play a key role in the localization of GABA(A) receptors in inhibitory synapses.Neuroplastins have two Ig domains that project into the extracellular space and are followed by a single 21 amino-acid membrane spanning sequence and a 34 amino-acid intracellular domain. The intracellular domain is mostly comprised of charged and neutral residues but also of a few hydrophobic residues. The N-terminus includes a 28 amino-acid signal peptide. In addition, neuroplastins contain a glutamate residue in their transmembrane domain which is important for molecular interactions within the membrane region.Although Np55 and Np65 are closely related in structure there are significant differences in terms of their expression. Np65 is tissue specific and is expressed mostly in forebrain neurons in the cortex, hippocampus and striatum. It has an anterior-posterior axis of expression. It is also expressed in lower amounts in the thalamus and hypothalamus. In contrast, Np55 is expressed throughout the brain in varying amounts.Currently, neuroplastins are not directly implicated in human diseases however several possible links have been discovered. A single nucleotide polymorphism (SNP) in the human neuroplastin locus is associated with reduced cortical thickness and impaired intellectual ability in adolescents. Furthermore, other SNPs in neuroplastin has been found to be either a risk or protecting factor for schizophrenia in animal models1.

Gen-ID: 56064

UniProt: P97546

Pathways: Regulation of long-term Neuronal Synaptic Plasticity