NrCAM Antikörper (Extracellular, N-Term)

Produktnummer ABIN7885176

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch NrCAM in WB, IHC, FACS und LCI. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für NrCAM Antikörper (Extracellular, N-Term) (ABIN7885176)

Target: NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NrCAM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Güteklasse: Carrier-free

Produktdetails anti-NrCAM Antikörper

Bindungsspezifität: AA 583-597, Extracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to NrCAM

Homologie: human - 13,16 amino acid residues identical,Mouse - 15

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)KDNGELPN DERFSVD, corresponding to amino acid residues 583-597 of rat NrCAM

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7236074)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW)

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu NrCAM

Target: NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))

Andere Bezeichnung: Neuronal cell adhesion molecule

Hintergrund:

Synonyms: Neuronal cell adhesion molecule, Nr-CAM, NgCAM-related cell adhesion molecule, Ankyrin-binding cell adhesion molecule, Neuronal surface protein Bravo, HBravo

Description: NrCAM (Neuron-glia related cell adhesion molecule) is a cell adhesion molecule that belongs to the immunoglobulin-class (Ig) of the L1 family. NrCAM promotes axon growth and repulsion through trans homophilic binding of extracellular domains and intracellular coupling of the conserved cytoplasmic domains to the actin cytoskeleton. This interaction occurs through ankyrin B and ezrin-radixin-moesin adaptors. In addition, NrCAM is responsible for controlling dendritic spine densities, axonal guidance and targeting, and neurite outgrowth by acting as a co-receptor molecule at the neuronal cell surface1,2.NrCAM is highly expressed in the cortex1,3. Its structure contains a PDZ binding motif with the potential to bind PSD scaffold proteins PSD-95 and SAP102. The Ig domains of the protein mediate homophilic and heterophilic binding to multiple ligands2,3.Genetic polymorphisms in the NrCAM gene are linked to autism spectrum disorders, intellectual disability and addictive behavior. NrCAM knockout mice demonstrate autism spectrum disorder related behaviors including impaired sociability, cognitive inflexibility, hypersensitivity to sensory stimuli and altered visual cortical responses.

Gen-ID: 497815

UniProt: P97686

Pathways: Regulation of Cell Size