MANF Antikörper (AA 91-104)

Produktnummer ABIN7885139

Der Kaninchen Polyklonal anti-MANF Antikörper (ABIN7885139) detektiert spezifisch MANF in WB, IHC und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für MANF Antikörper (AA 91-104) (ABIN7885139)

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MANF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Güteklasse: Carrier-free

Produktdetails anti-MANF Antikörper

Bindungsspezifität: AA 91-104

Verwendungszweck: A Rabbit Polyclonal Antibody to MANF

Homologie: Rat,mouse - identical

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)EVSKPLAHHIPVEK, corresponding to amino acid residues 91-104 of human MANF

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:600

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235952)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW)

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu MANF

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Andere Bezeichnung: Mesencephalic astrocyte-derived neurotrophic factor

Hintergrund:

Synonyms: Mesencephalic astrocyte-derived neurotrophic factor, Arginine-rich mutated in early stage tumors, ARP

Description: The novel protein mesencephalic astrocyte-derived neurotrophic factor (MANF) is a survival molecule (alternatively referred to as arginine-rich, mutated in early stage tumors, or ARMET) that has high selectivity for dopaminergic neurons of the midbrain1,2. There, it induces proliferative and protective effects against neurodegeneration (usually caused by 6-hydroxydopamine), its therapeutic potential in dopamine-related neurodegenerative disorders such as Parkinson's disease is therefore promising3,4.MANF is also expressed cardiac tissues. As was recently suggested, it induces cardioprotection in the heart and anti-hypertrophic effects via reducing endoplasmic reticulum stress in ischemic conditions in both the heart and the brain. Thus, its effects stretch beyond rescue and protection of dopaminergic neurons alone1,5.Generally, neurotrophic factors (NTFs) provide support, protection, and regulation for neurons - with each group of neurons having their own unique NTFs - both during development and at adulthood. Other noticeable NTFs include neurotrophin-3 and -4 (NT3 and NT4), nerve growth factor (NGF), brain derived neurotrophic factor (BDNF), and glial cell derived neurotrophic factor (GDNF)6.MANF is considered to be evolutionary conserved within Mammalia and beyond (including distant species such as nematode and fruit fly - the latter shares more than 50 % identity with human MANF gene) and is highly mutative in many tumors. It is mostly composed of alpha helices and random coils, its structure essentially differs from any other neurotrophic factor. It is suggested that the protein initiates intracellular signaling cascade by binding to cell-surface lipids and membranes via its cysteine-rich N-terminal. Together with its C-terminal which contains a disulfide bridge that endows a cytoprotective response against ER stress, MANF is implied to be bi-functional3,7.

Gen-ID: 7873

UniProt: P55145