GRPR Antikörper (3rd Extracellular Loop)

Produktnummer ABIN7885033

Der Kaninchen Polyklonal anti-GRPR Antikörper wird verwendet zum Nachweis von GRPR in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC, IF, IC und LCI.

Kurzübersicht für GRPR Antikörper (3rd Extracellular Loop) (ABIN7885033)

Target: GRPR (Gastrin-Releasing Peptide Receptor (GRPR))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GRPR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Güteklasse: Carrier-free

Produktdetails anti-GRPR Antikörper

Bindungsspezifität: 3rd Extracellular Loop, AA 287-300

Verwendungszweck: A Rabbit Polyclonal Antibody to Bombesin Receptor 2/Gastrin-releasing Peptide Receptor

Homologie: Rat,mouse,dog - identical

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)RSYHYSEVDTSMLH, corresponding to amino acid residues 287-300 of human BB2R 

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200-1:500

FC: The optimal concentration should be determined by the user

ICC: 1:100

IHC: 1:50

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235573)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW)

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu GRPR

Target: GRPR (Gastrin-Releasing Peptide Receptor (GRPR))

Andere Bezeichnung: BB2R

Hintergrund:

Synonyms: BB2R, Gastrin-releasing peptide receptor, GRP-preferring bombesin receptor

Description: Bombesin receptor 2 (BB2) is a member of a family of receptors that binds the 14 amino acid peptide, bombesin. Bombesin was first isolated from frog skin and it was later established that mammals express endogenous bombesin-like peptides such as gastrin-releasing peptide (GRP, a 27 amino acid homologue) and neuromedin B (NMB, a 10 amino acid homologue).BB2 is the preferred receptor for GRP and hence it is also referred to as GRP receptor or GRP-R.1 BB1 is the preferred receptor for NMB and therefore it is also known as NMB-R. A third member (called BB3) of the bombesin receptor family has been recognized but the endogenous mammalian ligand for this receptor remains to be identified.All three bombesin receptors are members of the seven-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. BB2 is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3), intracellular Ca2+ mobilization, and cell growth.2GRP (through its binding to BB2) plays an essential role in the release of hormones in the intestinal tract, the regulation of smooth muscle contraction, and the secretion of pancreatic enzymes.2 In the brain, BB2 has been implicated in regulating feeding behavior, thermoregulation, and memory formation.3Finally, BB2 is overexpressed in several human tumors including breast, prostate, and lung, where it acts as a growth factor receptor inducing tumor growth. BB2 is considered a potential target for the development of both diagnostics and anti-cancer therapies.4

Gen-ID: 2925

UniProt: P30550