GPR4 Antikörper (Extracellular Loop)

Produktnummer ABIN7884985

Der Kaninchen Polyklonal anti-GPR4 Antikörper wird verwendet zum Nachweis von GPR4 in Proben von Human, Ratte und Maus. Er wurde validiert für WB und IHC.

Kurzübersicht für GPR4 Antikörper (Extracellular Loop) (ABIN7884985)

Target: GPR4 (G Protein-Coupled Receptor 4 (GPR4))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Güteklasse: Carrier-free

Produktdetails anti-GPR4 Antikörper

Bindungsspezifität: AA 162-177, Extracellular Loop

Verwendungszweck: A Rabbit Polyclonal Antibody to GPR4

Homologie: Rat,mouse,human - 15,16 amino acid residues identical.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)RDRYNHTF(S)FEKFPME, corresponding to amino acid residues 162-177 of rat GPR4

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:400

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235415)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu GPR4

Target: GPR4 (G Protein-Coupled Receptor 4 (GPR4))

Andere Bezeichnung: GPR4

Hintergrund:

Synonyms: Acid-sensing G-protein coupled receptor 4, GPR19

Description: G-protein coupled receptor 4 (GPR4) belongs to a protein family comprised of 3 related G-protein coupled receptors (GPCRs): GPR4, OGR1/GPR68 and TDAG8/GPR65. Like all GPCRs, GPR4's structure is composed of seven-transmembrane-spanning domains linked by an N-terminal extracellular domain and a C-terminal intracellular domain2.GPR4 responds to pH changes and is activated by a physiological increase in extracellular H+ through several signaling pathways including- the G12/13-protein/Rho, the Gq/PLC, and the Gs-protein/cAMP pathway1,2. It is fully activated at pH levels of 6.8 and lower and is inactive at pH levels of 7.5 and higher3,4.Recent studies show that the pH -sensing GPCRs also have the ability to regulate cancer cell metastasis and proliferation, immune cell function, inflammation, blood vessel formation, endothelial barrier function thus making them an important therapeutic target for different diseases. Overexpression of GPR4 in human cancer cells shows involvement in driving or maintaining tumor formation.GPR4 is expressed in several tissues including lung, vascular endothelial cells, kidney, heart, liver, skeletal muscle, ovary, and placenta.

Gen-ID: 308408

UniProt: Q4KLH9