Ephrin A2 Antikörper (Extracellular)

Produktnummer ABIN7884943

Der Kaninchen Polyklonal anti-Ephrin A2 Antikörper wird verwendet zum Nachweis von Ephrin A2 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IF, FACS, IHC und LCI.

Kurzübersicht für Ephrin A2 Antikörper (Extracellular) (ABIN7884943)

Target: Ephrin A2 (EFNA2)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ephrin A2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Live Cell Imaging (LCI)

Güteklasse: Carrier-free

Produktdetails anti-Ephrin A2 Antikörper

Bindungsspezifität: AA 71-84, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to Ephrin-A2

Homologie: Rat - identical, human - 13,14 amino acid residues identical

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)HYGAPLPPAERMER, corresponding to amino acid residues 71-84 of mouse Ephrin-A2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:400

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235305)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW)

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu Ephrin A2

Target: Ephrin A2 (EFNA2)

Andere Bezeichnung: EPH-related receptor tyrosine kinase ligand 6

Hintergrund:

Synonyms: EPH-related receptor tyrosine kinase ligand 6, LERK6, CEK7-ligand, EFNA2

Description: Eph receptor tyrosine kinases family and their ligands, ephrins, are membrane proteins that primarily regulate cell to cell repulsion and cell adhesion and movement by modulating the organization of the actin cytoskeleton mainly through Rho family GTPases. Eph receptors and ephrin ligand signaling regulaes many aspects of cell interaction in the developing neocortex, including migration and axonal target recognition. Ephrin signaling plays an important role in guiding neurons and their projections during embryonic development1,2.In mammals, the Eph receptor tyrosine kinases family has 14 members that are divided into 2 subclasses, the EphA (A1-A8 and A10) and EphB (B1-B4 and B6). This division is based on the sequence similarity of their extracellular domains. EphA receptors bind to glycosylphosphatidyl inositol-anchored ephrin-A ligands, and EphB receptors bind to transmembrane ephrin-B proteins. The ephrin-A proteins include five members ephrin-A1 to ephrin-A5 whereas ephrin-B proteins include three ephrin-B1 to ephrin-B32,3.Ephrin-A2 protein is abundantly expressed in retinal axons. Mutations in Ephrin-A2 disrupts topographic ordering of projections in the developing visual system. A knockout of ephrin-A2 in adult mice shows focal patches of disorganized neocortical laminar architecture, ranging in severity from reduced neuronal density to a complete lack of neurons1,2.

Gen-ID: 13637

UniProt: P52801