Contactin 1 Antikörper (Extracellular, N-Term)

Produktnummer ABIN7884895

Der Kaninchen Polyklonal anti-Contactin 1 Antikörper wird verwendet zum Nachweis von Contactin 1 in Proben von Human, Ratte und Maus. Er wurde validiert für WB, IHC und IF.

Kurzübersicht für Contactin 1 Antikörper (Extracellular, N-Term) (ABIN7884895)

Target: Contactin 1 (CNTN1)

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Contactin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Güteklasse: Carrier-free

Produktdetails anti-Contactin 1 Antikörper

Bindungsspezifität: AA 947-961, Extracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to Contactin-1 (extracellular)

Homologie: Mouse - identical, human - 14,15 amino acid residues identical

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)RPDGQHDGKLFSTHK, corresponding to amino acid residues 947 - 961 of rat Contactin-1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200-1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235161)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW)

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu Contactin 1

Target: Contactin 1 (CNTN1)

Andere Bezeichnung: Neural cell surface protein F3

Hintergrund:

Synonyms: Neural cell surface protein F3, Glycoprotein GP135, CNTN1

Description: Contactin-1 (CNTN1), also known as F3/Contactin a cell adhesion molecule, is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein involved in neuronal development, synaptic maintenance and organization of neuronal networks. CNTNs are a subpopulation of molecules belonging to the immunoglobulin (Ig) superfamily, which includes six members: CNTN1, TAG-1/CNTN2, BIG-1/CNTN3, BIG-2/CNTN4, NB-2/CNTN5 and NB-3/CNTN61,2.In mammals, contactin-1 is mostly localized in the peripheral and central neural system. It is highly expressed at the axonal level and mediates various functions in both glial and neuronal cells, it is involved in neuronal and glial development and differentiation, myelination, synaptogenesis and synaptic function1.Contactin-1 expression profile differs in various brain regions and, within each area, it changes depending upon the cell type. For example, in the cerebellar cortex contactin-1 is predominant in granule cells in earlier developmental stages, whereas its activation is delayed in Purkinje neurons. In the hippocampus, it is detected on pyramidal neurons of CA1-CA3 fields and in granule layers of Dentate Gyrus (DG) where its expression persists in postnatally developed neurons. Hippocampal contactin-1 is highly expressed in the developing hippocampus, it progressively increases during postnatal life, but it declines during aging, paralleling cognitive impairment. This suggests that contactin-1 might play a role in memory processes, in line with studies indicating its involvement in neurotransmitter release and some forms of plasticity1.The role of contactin-1 in neurotransmitter release has been linked with its capability to regulate synaptic vesicle exocytosis. Contactin-1 overexpression had been shown to induce an increase of cAMP-Responsive Element Binding (CREB) phosphorylation, enhanced hippocampal-dependent LTP and memory especially in 12-months-old mice, where neurogenesis is particularly evident. Also, contactin-1 overexpression counteracts apoptosis, and induces an increase of the antiapoptotic- and neuroplasticity-pathway BDNF/CREB/Bcl-21,3.Contactin-1 plays an important role in cancer progression. The expression of contactin-1 is upregulated in primary lesions, and its expression level correlates with tumor metastasis in cancer patients. Studies have demonstrated that contactin-1 also plays a key role in diseases not related to the nervous system, and its most notable function is its participation in cancer progression, in cancers such as esophageal squamous cell carcinoma (ESCC), GC, lung adenocarcinoma, oral squamous cell carcinoma (OSCC), hepatocellular carcinoma and prostate cancer2,3.

Gen-ID: 117258

UniProt: Q63198