CHERP Antikörper (Cytosolic)

Produktnummer ABIN7884835

Der Kaninchen Polyklonal anti-CHERP Antikörper wird verwendet zum Nachweis von CHERP in Proben von Maus und Ratte. Er wurde validiert für WB, IHC und IF.

Kurzübersicht für CHERP Antikörper (Cytosolic) (ABIN7884835)

Target: CHERP (Calcium Homeostasis Endoplasmic Reticulum Protein (CHERP))

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CHERP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Güteklasse: Carrier-free

Produktdetails anti-CHERP Antikörper

Bindungsspezifität: AA 669-684, Cytosolic

Verwendungszweck: A Rabbit Polyclonal Antibody to CHERP

Homologie: Rat,human - identical

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)KLEDHEYKPLDPKDIR, corresponding to amino acid residues 669-684 of mouse CHERP

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:1500

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235001)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW)

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu CHERP

Target: CHERP (Calcium Homeostasis Endoplasmic Reticulum Protein (CHERP))

Andere Bezeichnung: Calcium homeostasis endoplasmic reticulum protein

Hintergrund:

Synonyms: Calcium homeostasis endoplasmic reticulum protein, SR-related CTD-associated factor 6, SCAF6

Description: CHERP (calcium homeostasis endoplasmic reticulum protein) is a ubiquitously expressed integral sarco/endoplasmic reticulum (SR/ER) membrane protein. CHERP protein consist of 916 amino acid in humans and its structure containing a Pro-rich region and a segment of Arg-Ser dipeptide repeats (an RS domain) near the C terminus, and an RNA recognition motifs (RRMs)1-3.CHERP acts as a regulator of both major families of endoplasmic reticulum Ca2+ channels, inositol 1,4,5-trisphosphate receptors (IP3Rs) and ryanodine receptors (RyRs). CHERP is responsible for the regulation of intracellular Ca2+ mobilization in human cells and is involved in neuroblastoma cell proliferation, apoptosis and tumorigenicity1,2.CHERP co-localizes with IP3 receptors throughout the cytoplasmic and perinuclear regions in human erythroleukemia cells and in Jurkat cells. CHERP is also detected in the nucleoplasm with prominent accumulation at nuclear speckles1,2. CHERP co-localizes with endogenous ryanodine receptor type 1 (RyR1) in the sarcoplasmic reticulum of rat soleus muscle. Studies of CHERP knockdown reveal decreased intracellular Ca2+ mobilization, cell growth and proliferation2,3.

Gen-ID: 27967

UniProt: Q8CGZ0