ADAM22 Antikörper (Extracellular, N-Term)

Produktnummer ABIN7884717

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch ADAM22 in WB, IHC und IF. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für ADAM22 Antikörper (Extracellular, N-Term) (ABIN7884717)

Target: ADAM22 (ADAM Metallopeptidase Domain 22 (ADAM22))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ADAM22 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Güteklasse: Carrier-free

Produktdetails anti-ADAM22 Antikörper

Bindungsspezifität: AA 715-729, Extracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to ADAM22

Homologie: Rat,human - identical

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)HNDDAKTGITLSGNG, corresponding to amino acid residues 715-729 of mouse ADAM22

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7234619)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW)

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu ADAM22

Target: ADAM22 (ADAM Metallopeptidase Domain 22 (ADAM22))

Andere Bezeichnung: Disintegrin and metalloproteinase domain-containing protein 22

Hintergrund:

Synonyms: Disintegrin and metalloproteinase domain-containing protein 22, ADAM metallopeptidase domain 22, MDC2

Description: ADAMs (a disintegrin and metalloproteinases) are multi-domain transmembrane glycoproteins with diverse roles in physiology and disease. Notably, 8 of 21 ADAMs lack functional metalloproteinase domains and are implicated in protein-protein interactions instead of membrane protein ectodomain shedding.ADAM22 is a non-proteinase which acts as a post synaptic receptor for the secreted neurotransmission modulator LGI-1 at neural synapses. ADAM22 is a compact four-leaf clover with the metalloproteinase-like domain (Domain M) held in the concave face of a rigid module formed by the disintegrin (Domain D), cysteine-rich (Domain C), and epidermal growth factor-like domains (Domain E). The largest domain in the four-leaf clover, domain M, is distal to the cell membrane. Following domain M, domain D and domain C zigzag to domain E in a compact, but not extended fashion. A 15-amino acid linker, leads the C terminus of domain E to the membrane. The loss of metalloproteinase activity is ensured by the absence of critical catalytic residues, the filling of the substrate groove, and the steric hindrance by the cysteine-rich domain. The extracellular domain of ADAM22 interacts with LGI-1, whereas its cytoplasmic PDZ-binding motif recruits PSD-95. The link of ADAM22 and LGI-1 to AMPA receptors establishes their roles in glutamate neurotransmission1.Mutations that impair LGI-1 binding to ADAM22 are implicated in the pathogenesis of Autosomal dominant lateral temporal epilepsy (ADTLE), a focal epilepsy syndrome characterized by focal seizures with prominent auditory or aphasic symptoms, normal magnetic resonance imaging, and usually benign evolution2.

Gen-ID: 11496

UniProt: Q9R1V6