DBH Antikörper

Produktnummer ABIN7884240

Dieser Maus Monoklonal Antikörper detektiert spezifisch DBH in WB, IHC, ELISA und ICC. Es zeigt Reaktivität gegenüber Proben von Ratte.

Kurzübersicht für DBH Antikörper (ABIN7884240)

Target: DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser DBH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Klon: DBH41

Produktdetails anti-DBH Antikörper

Verwendungszweck: Dopamine Beta Hydroxylase (DBH) Mouse Monoclonal Antibody (DBH41)

Spezifität: This antibody recognizes This antibody specifically recognizes two bands of molecular weight 70 and 75 kDa, corresponding to the soluble and membrane bound forms of the enzyme, respectively. Due to the high sensitivity of the DBH antibody, the presence of the enzyme can be immunologically detected even in areas where only scattered DBH-containing fibers are present. DBH antibody specifically stains DBH-containing neurons and fibers in the rat central nervous system (CNS). It exhibits no cross-reactivity with either mouse, human, rabbit, guinea pig, cat or bovine DBH

Immunogen: Rat DBH

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: If reconstituted with deionized water in 2 ml: IHC and ICC: 1:30 - 1:50. Optimal dilution has to be determined by the user.

Buffer: Lyophilized tissue culture supernatant

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Haltbarkeit: 24 months

Details zu DBH

Target: DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))

Andere Bezeichnung: Dopamine Beta Hydroxylase

Hintergrund:

Dopamine beta-monooxygenase Dopamine Beta Hydroxylase, Dopamine-hydroxylase, DBH, oxygenase, anti-Dopamine Beta Hydroxylase, anti-Dopamine-hydroxylase, anti-DBH, oxygenase

Studying the monoaminergic system has been of great interest and importance since dopamine, noradrenaline and adrenaline are the neurotransmitters involved in multiple physiological and pathophysiological conditions. DBH is the enzyme responsible for the biosynthetic hydroxylation of dopamine to noradrenaline.

Pathways: Carbohydrate Homeostasis