TYR Antikörper

Produktnummer ABIN7882606

Der Maus Monoklonal anti-TYR Antikörper wird verwendet zum Nachweis von TYR in Proben von Human. Er wurde validiert für IHC (p).

Kurzübersicht für TYR Antikörper (ABIN7882606)

Target: TYR (Tyrosinase (TYR))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TYR Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: SPM360

Produktdetails anti-TYR Antikörper

Verwendungszweck: Tyrosinase Antibody

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant tyrosinase protein was used as the immunogen for this Tyrosinase antibody.

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the Tyrosinase antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, or 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Tyrosinase antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu TYR

Target: TYR (Tyrosinase (TYR))

Andere Bezeichnung: Tyrosinase

Hintergrund: Recognizes a cluster of proteins between 70-80 kDa, identified as tyrosinase. Occasionally a minor band at 55 kDa is also detected. This mAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway, the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this mAb shows tyrosinase in melanotic as well as amelanotic variants. This mAb is a useful marker for melanocytes and melanomas.

UniProt: P14679