Rekombinanter NSDHL Antikörper

Produktnummer ABIN7881756

Dieser Kaninchen Monoklonal Antikörper detektiert spezifisch NSDHL in WB und IP. Es zeigt Reaktivität gegenüber Proben von Human, Ratte und Maus.

Kurzübersicht für Rekombinanter NSDHL Antikörper (ABIN7881756)

Target: NSDHL (NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser NSDHL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Klon: 30N66

Produktdetails anti-NSDHL Antikörper

Verwendungszweck: NSDHL Antibody / NADP-dependent steroid dehydrogenase-like protein

Aufreinigung: Affinity chromatography

Immunogen: A synthesized peptide derived from human NSDHL was used as the immunogen for the NSDHL antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the NSDHL antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store the NSDHL antibody at -20oC.

Details zu NSDHL

Target: NSDHL (NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL))

Andere Bezeichnung: NSDHL

Hintergrund: NSDHL antibody is designed to detect NAD(P)-dependent steroid dehydrogenase-like protein, an essential enzyme of the cholesterol biosynthetic pathway. NSDHL is localized to the endoplasmic reticulum membrane and functions as a C-4 sterol dehydrogenase, catalyzing the removal of methyl groups from sterol intermediates. This enzymatic activity is necessary for producing cholesterol and other sterols that are critical for membrane structure, signaling, and developmental processes.

NSDHL antibody is widely used in studies of metabolism, developmental biology, and genetic disorders. Mutations in the NSDHL gene are associated with CHILD syndrome (congenital hemidysplasia with ichthyosiform erythroderma and limb defects), an X-linked dominant disorder characterized by skin abnormalities and limb malformations. Detecting NSDHL with specific antibodies enables researchers to evaluate expression and function in disease models and clinical studies.

Applications of NSDHL antibody include western blotting, immunohistochemistry, and immunofluorescence. In western blot assays, NSDHL antibody detects the protein with high specificity, confirming its expression across cell and tissue samples. Immunohistochemistry reveals distribution patterns in tissues involved in sterol metabolism, while immunofluorescence highlights its endoplasmic reticulum localization in cultured cells. These experimental approaches provide important insights into cholesterol biosynthesis at the molecular level.

NSDHL plays a vital role in cholesterol metabolism, and dysregulation of its activity has broader implications for human health. Abnormal sterol accumulation contributes to metabolic disease, cardiovascular dysfunction, and neurodevelopmental abnormalities. Using NSDHL antibody, researchers can track enzyme activity and explore how disruptions in cholesterol biosynthesis impact cellular and systemic function.

Beyond inherited disorders, NSDHL has been implicated in cancer biology. Cholesterol metabolism supports rapid tumor growth, and enzymes in the biosynthetic pathway are increasingly recognized as potential therapeutic targets. Monitoring NSDHL expression with antibodies allows investigators to connect sterol metabolism with oncogenic processes, highlighting new avenues for intervention.

NSDHL antibody from NSJ Bioreagents is a reliable reagent for studying sterol metabolism, developmental genetics, and cholesterol-related disease. Its specificity ensures accurate detection in multiple research settings, supporting basic and translational studies alike.

UniProt: Q15738