NDST1 Antikörper

Produktnummer ABIN7881747

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch NDST1 in WB und ELISA. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für NDST1 Antikörper (ABIN7881747)

Target: NDST1 (N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 1 (NDST1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NDST1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-NDST1 Antikörper

Verwendungszweck: NDST1 Antibody / N-deacetylase/N-sulfotransferase 1

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human NDST1 recombinant protein (Position: 40-87) was used as the immunogen for the NDST1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the NDST1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the NDST1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu NDST1

Target: NDST1 (N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 1 (NDST1))

Andere Bezeichnung: NDST1

Hintergrund: NDST1 antibody detects N-deacetylase/N-sulfotransferase 1, an essential enzyme involved in the biosynthesis of heparan sulfate, a glycosaminoglycan that modulates cell signaling, adhesion, and extracellular matrix organization. NDST1 catalyzes two critical reactions-N-deacetylation and N-sulfation of glucosamine residues-forming the sulfated domains that give heparan sulfate its functional specificity. The NDST1 antibody is widely used in glycobiology, developmental, and cancer research to study proteoglycan biosynthesis, cell-matrix interactions, and signaling regulation.

NDST1 is encoded by the NDST1 gene located on human chromosome 5q33.1. The protein is approximately 882 amino acids long and resides in the lumen of the Golgi apparatus, where it acts early in heparan sulfate chain modification. NDST1 belongs to the heparan sulfate modification enzyme family, working in concert with other sulfotransferases and epimerases to establish specific sulfation patterns that control the binding affinity of heparan sulfate to growth factors, morphogens, and cytokines.

The NDST1 antibody detects a 100 kilodalton band by western blot and demonstrates perinuclear Golgi localization under immunofluorescence microscopy. NDST1 activity is essential for embryonic development, vascular formation, and neural patterning. Through its modification of heparan sulfate, NDST1 regulates key signaling pathways including FGF, Wnt, Hedgehog, and BMP. Loss or mutation of NDST1 disrupts heparan sulfate composition, leading to developmental abnormalities and impaired growth factor signaling.

In cancer, NDST1 expression is often dysregulated, resulting in altered extracellular matrix structure and aberrant cell signaling. Overexpression enhances tumor invasiveness by modifying cell-surface heparan sulfate, while reduced NDST1 activity interferes with angiogenesis. It also contributes to inflammation by modulating chemokine gradients and leukocyte migration.

Because NDST1 determines the sulfation code of heparan sulfate, it serves as a critical regulator of cell communication and extracellular architecture. NSJ Bioreagents provides a validated NDST1 antibody optimized for its applications, supporting investigations into glycosaminoglycan biosynthesis, developmental regulation, and tumor microenvironment signaling.

UniProt: P52848

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Glycosaminoglycan Metabolic Process