MUC5AC Antikörper

Produktnummer ABIN7881678

Dieser Maus Monoklonal Antikörper detektiert spezifisch MUC5AC in IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für MUC5AC Antikörper (ABIN7881678)

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MUC5AC Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: SPM297

Produktdetails anti-MUC5AC Antikörper

Verwendungszweck: Mucin-5AC Antibody

Aufreinigung: Protein G affinity chromatography

Immunogen: An M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient was used as the immunogen for this Mucin-5AC antibody.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the Mucin-5AC antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Mucin-5AC antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu MUC5AC

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Andere Bezeichnung: Mucin-5AC

Hintergrund: This mAb recognizes the peptide core of gastric mucin M1 (>1,000 kDa) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.

UniProt: P98088