Rekombinanter IQGAP2 Antikörper

Produktnummer ABIN7881328

Der Kaninchen Monoklonal anti-IQGAP2 Antikörper wird verwendet zum Nachweis von IQGAP2 in Proben von Human. Er wurde validiert für WB und IHC.

Kurzübersicht für Rekombinanter IQGAP2 Antikörper (ABIN7881328)

Target: IQGAP2 (IQ Motif Containing GTPase Activating Protein 2 (IQGAP2))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser IQGAP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: 30I08

Produktdetails anti-IQGAP2 Antikörper

Verwendungszweck: IQGAP2 Antibody / IQ motif containing GTPase activating protein 2

Aufreinigung: Affinity-chromatography

Immunogen: A synthesized peptide derived from human IQGAP2 was used as the immunogen for the IQGAP2 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the IQGAP2 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store the IQGAP2 antibody at -20oC.

Details zu IQGAP2

Target: IQGAP2 (IQ Motif Containing GTPase Activating Protein 2 (IQGAP2))

Andere Bezeichnung: IQGAP2

Hintergrund: IQGAP2 antibody detects IQ motif containing GTPase activating protein 2, encoded by the IQGAP2 gene. IQGAP2 belongs to a family of scaffolding proteins that integrate cytoskeletal dynamics, cell adhesion, and intracellular signaling. Unlike classical GTPase activating proteins, IQGAPs stabilize the active GTP bound form of small GTPases such as Rac1 and Cdc42. Through its IQ motifs, calponin homology domain, and RasGAP related domain, IQGAP2 coordinates actin reorganization, cell polarity, and signaling cascades.

IQGAP2 antibody is widely used in cancer biology, hepatology, and cytoskeletal research. IQGAP2 is highly expressed in liver, stomach, and testis. It regulates actin dynamics and adherens junctions by interacting with beta catenin, E cadherin, and F actin. Altered expression of IQGAP2 has been linked to hepatocellular carcinoma and gastric cancer, where loss of IQGAP2 promotes tumorigenesis. By detecting IQGAP2, researchers can study its tumor suppressor functions and its role in epithelial tissue integrity.

Western blot assays with IQGAP2 antibody detect protein bands in hepatic and epithelial tissues. Immunohistochemistry highlights expression in liver lobules and gastric mucosa, while immunofluorescence reveals cytoplasmic localization at cell junctions and actin filaments. ELISA enables quantification of IQGAP2 expression across experimental samples.

IQGAP2 participates in multiple signaling pathways, including Wnt, MAPK, and PI3K AKT. Its ability to bind both actin and signaling proteins positions it as a key scaffold at the plasma membrane. In cancer, IQGAP2 expression is frequently downregulated, contrasting with IQGAP1, which is often upregulated. This differential regulation underscores the unique tumor suppressive properties of IQGAP2. By applying IQGAP2 antibody, scientists can investigate how cytoskeletal regulation intersects with cancer biology and metabolic homeostasis.

IQGAP2 also plays roles in immune signaling and reproduction. In immune cells, it modulates cytoskeletal dynamics during migration and activation. In testis, IQGAP2 expression suggests involvement in spermatogenesis and germ cell polarity. These diverse roles make IQGAP2 antibody a valuable reagent for broad research areas beyond oncology.

IQGAP2 antibody from NSJ Bioreagents provides dependable specificity for studying cytoskeletal scaffolding and tumor suppression. Its strong performance across techniques ensures accurate detection of IQGAP2 in multiple research contexts.

UniProt: Q13576