Histone H1 Antikörper

Produktnummer ABIN7881067

Der Maus Monoklonal anti-Histone H1 Antikörper (Klon SPM256) (ABIN7881067) detektiert spezifisch Histone H1 in IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für Histone H1 Antikörper (ABIN7881067)

Target: Histone H1

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Histone H1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: SPM256

Produktdetails anti-Histone H1 Antikörper

Verwendungszweck: Anti-Histone H1 Antibody

Aufreinigung: Protein G affinity chromatography

Immunogen: Nuclei of human leukemia biopsy cells were used as the immunogen for the anti-Histone H1 antibody.

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the anti-Histone H1 antibody should be determined by the researcher.

1. Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the anti-Histone H1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu Histone H1

Target: Histone H1

Hintergrund: Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer, formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80 % of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

UniProt: P07305