CA9 Antikörper

Produktnummer ABIN7880170

Der Maus Monoklonal anti-CA9 Antikörper (Klon SPM314) (ABIN7880170) detektiert spezifisch CA9 in IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für CA9 Antikörper (ABIN7880170)

Target: CA9 (Carbonic Anhydrase IX (CA9))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CA9 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: SPM314

Produktdetails anti-CA9 Antikörper

Verwendungszweck: CA9 Antibody

Aufreinigung: Protein G affinity chromatography

Immunogen: The microsomal fraction of human renal cortical tissue homogenate was used as the immunogen for this CA9 antibody.

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the CA9 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the CA9 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu CA9

Target: CA9 (Carbonic Anhydrase IX (CA9))

Andere Bezeichnung: CA9

Hintergrund: Recognizes a glycoprotein of ~200 kDa, identified as carbonic anhydrase IX (CAIX/CA9/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, CA9 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, CA9 is expressed by 93 % of primary and 84 % of metastatic renal cell carcinomas. This mAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

UniProt: Q16790