Rekombinanter Biotin Antikörper
Kurzübersicht für Rekombinanter Biotin Antikörper (ABIN7880080)
Target
Antikörpertyp
Wirt
Klonalität
Konjugat
Applikation
Güteklasse
Klon
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Verwendungszweck
- Recombinant Biotin Antibody / Rabbit Monoclonal (azide and preservative free)
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Aufreinigung
- Protein A affinity chromatography
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Immunogen
- Biotinylated sheep immunoglobulin was used as the immunogen for this recombinant Biotin antibody.
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Isotyp
- IgG, kappa
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Applikationshinweise
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The optimal dilution of the recombinant Biotin antibody for each application should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1 mg/mL
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Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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Konservierungsmittel
- Azide free
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store the recombinant Biotin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- Biotin
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Substanzklasse
- Chemical
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Hintergrund
- This mAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Target
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