ANO1 Antikörper

Produktnummer ABIN7879983

Der Maus Monoklonal anti-ANO1 Antikörper wird verwendet zum Nachweis von ANO1 in Proben von Human. Er wurde validiert für IHC (p).

Kurzübersicht für ANO1 Antikörper (ABIN7879983)

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ANO1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: SPM580

Produktdetails anti-ANO1 Antikörper

Verwendungszweck: ANO1 Antibody / DOG1 / TMEM16A

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant human DOG1 protein was used as the immunogen for this ANO1 antibody.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the ANO1 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the ANO1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu ANO1

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Andere Bezeichnung: ANO1

Hintergrund: Expression of DOG-1/ANO1/TMEM16A protein is elevated in the gastrointestinal stromal tumors (GISTs), c-kit signaling-driven mesenchymal tumors of the GI tract. ANO1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity has been reported in 97.8 percent of scorable GISTs, including all c-kit negative GISTs. Overexpression has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of DOG-1/ANO1 and c-kit in GISTs is nearly identical: 94.4 % vs. 94.7 %.

UniProt: Q5XXA6