Rekombinanter PBK Antikörper (pThr9)

Produktnummer ABIN7879809

Der Kaninchen Monoklonal anti-PBK Antikörper wird verwendet zum Nachweis von PBK in Proben von Human. Er wurde validiert für WB und IHC.

Kurzübersicht für Rekombinanter PBK Antikörper (pThr9) (ABIN7879809)

Target: PBK (PDZ Binding Kinase (PBK))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser PBK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: 30P33

Produktdetails anti-PBK Antikörper

Bindungsspezifität: pThr9

Verwendungszweck: Phospho-PBK (Thr9) Antibody / PDZ binding kinase

Aufreinigung: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Phospho-PBK/TOPK (Thr9) was used as the immunogen for the Phospho-PBK (Thr9) Antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Phospho-PBK (Thr9) Antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store the Phospho-PBK (Thr9) Antibody at -20oC.

Details zu PBK

Target: PBK (PDZ Binding Kinase (PBK))

Andere Bezeichnung: PBK

Hintergrund: Phospho-PBK (Thr9) antibody detects PDZ binding kinase, also known as lymphokine activated killer T cell origin protein kinase (TOPK), when phosphorylated at threonine 9. PBK is encoded by the PBK gene and belongs to the MAP kinase kinase family. It is highly expressed in proliferating cells, especially in testis, placenta, and activated lymphocytes. Phosphorylation at Thr9 regulates PBK kinase activity, influencing cell cycle progression and mitotic events. This makes Phospho-PBK (Thr9) antibody a key tool for studying proliferative signaling and tumor biology.

Phospho-PBK (Thr9) antibody is widely applied in oncology, immunology, and cell cycle research. PBK activation supports G2/M transition by phosphorylating downstream substrates involved in spindle formation and chromosome segregation. It also participates in immune signaling pathways in T cells and NK cells. By detecting phosphorylation at Thr9, researchers can monitor PBK activation and its role in proliferation and immunity.

Applications for Phospho-PBK (Thr9) antibody include western blotting, immunohistochemistry, and immunofluorescence. Western blot assays detect phosphorylated PBK isoforms, immunohistochemistry maps expression in tumors, and immunofluorescence reveals subcellular localization at centrosomes during mitosis. These complementary approaches provide detailed evaluation of PBK biology.

Dysregulated PBK activity is implicated in multiple cancers, including lung, breast, and hematologic malignancies. Elevated Thr9 phosphorylation correlates with tumor grade and poor prognosis, reflecting hyperactive proliferation. In immune biology, PBK phosphorylation regulates lymphocyte activation, linking cell cycle control to host defense. By applying Phospho-PBK (Thr9) antibody, scientists can study these dual roles in cancer and immunity.

Therapeutic research has targeted PBK as a potential kinase for inhibition. Small molecule inhibitors reduce proliferation and sensitize tumor cells to chemotherapy. Monitoring Thr9 phosphorylation provides a pharmacodynamic readout of drug activity. Phospho-PBK (Thr9) antibody from NSJ Bioreagents offers strong specificity for this site specific modification, supporting mechanistic and translational research into kinase biology.

UniProt: Q96KB5