Rekombinanter TBC1D4 Antikörper (pThr642)

Produktnummer ABIN7879804

Der Kaninchen Monoklonal anti-TBC1D4 Antikörper (Klon 31T47) (ABIN7879804) detektiert spezifisch TBC1D4 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Rekombinanter TBC1D4 Antikörper (pThr642) (ABIN7879804)

Target: TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser TBC1D4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: 31T47

Produktdetails anti-TBC1D4 Antikörper

Bindungsspezifität: pThr642

Verwendungszweck: Phospho-AS160 (Thr642) Antibody / TBC1D4

Aufreinigung: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Phospho-AS160 (T642) was used as the immunogen for the Phospho-AS160 (Thr642) antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Phospho-AS160 (Thr642) antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store the Phospho-AS160 (Thr642) antibody at -20oC.

Details zu TBC1D4

Target: TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))

Andere Bezeichnung: AS160

Hintergrund: Phospho-AS160 (Thr642) antibody detects TBC1 domain family member 4 when phosphorylated at threonine 642. This protein is encoded by the TBC1D4 gene and is commonly referred to as AS160, Akt substrate of 160 kDa, and insulin-regulated Rab GTPase-activating protein. TBC1D4 functions as a Rab GAP that regulates trafficking of GLUT4 vesicles in response to insulin signaling. Phosphorylation at Thr642 by Akt relieves its inhibitory GAP activity, allowing Rab GTPases to promote translocation of GLUT4 to the plasma membrane, a critical event in glucose uptake by adipocytes and muscle cells.

Phospho-AS160 (Thr642) antibody is widely applied in metabolism, endocrinology, and diabetes research. Detecting phosphorylation at Thr642 provides a direct readout of insulin-stimulated Akt signaling and GLUT4 trafficking. This makes the antibody valuable for studies of insulin resistance, obesity, and type 2 diabetes. In addition, TBC1D4 is expressed in other tissues, linking it to energy homeostasis beyond classical insulin-responsive cells.

Applications for Phospho-AS160 (Thr642) antibody include western blotting, immunohistochemistry, immunofluorescence, and ELISA. Western blot assays detect phosphorylation changes in response to insulin or exercise, immunohistochemistry maps expression in metabolic tissues, and immunofluorescence highlights vesicle trafficking events. These methods allow researchers to connect molecular signaling to physiological glucose uptake.

Dysregulation of TBC1D4 phosphorylation is linked to insulin resistance and diabetes. Reduced phosphorylation impairs GLUT4 trafficking, while mutations in TBC1D4 cause severe postprandial hyperinsulinemia and muscle glycogen storage abnormalities. By applying Phospho-AS160 (Thr642) antibody, scientists can study both common and rare metabolic disorders.

Beyond metabolism, TBC1D4 regulates vesicle trafficking in immune cells, neurons, and other systems. Phosphorylation-dependent regulation highlights its integration with Akt signaling in multiple contexts. NSJ Bioreagents provides Phospho-AS160 (Thr642) antibody with validated specificity, ensuring accurate detection of this phosphorylation event in insulin and trafficking research.

UniProt: O60343