ODF1 Antikörper (N-Term)

Produktnummer ABIN7879639

Der Kaninchen Polyklonal anti-ODF1 Antikörper (ABIN7879639) detektiert spezifisch ODF1 in WB und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für ODF1 Antikörper (N-Term) (ABIN7879639)

Target: ODF1 (Outer Dense Fiber of Sperm Tails 1 (ODF1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ODF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Produktdetails anti-ODF1 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: ODF1 Antibody / Outer dense fiber protein 1

Aufreinigung: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human ODF1 was used as the immunogen for the ODF1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the ODF1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the ODF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu ODF1

Target: ODF1 (Outer Dense Fiber of Sperm Tails 1 (ODF1))

Andere Bezeichnung: ODF1

Hintergrund: ODF1 antibody detects Outer dense fiber protein 1, encoded by the ODF1 gene on chromosome 8q22.2. ODF1 antibody is widely applied in reproductive biology, spermatogenesis, and fertility research. ODF1 is a structural protein of the sperm tail, specifically localized to the outer dense fibers that run alongside the axoneme in the principal piece of the flagellum. These fibers provide mechanical strength, protect the axoneme from shear stress, and contribute to the elastic recoil properties of sperm movement.

Structurally, ODF1 is a ~27 kDa coiled-coil protein with leucine zipper motifs that promote oligomerization. It is one of the earliest proteins incorporated into outer dense fibers during spermatid differentiation. ODF1 interacts with other outer dense fiber proteins such as ODF2 and with axonemal microtubules, forming the cytoskeletal backbone of the sperm tail.

Functionally, ODF1 contributes to sperm motility by reinforcing the axoneme and supporting energy transmission along the flagellum. Loss of ODF1 leads to structural abnormalities in the sperm tail and impaired motility, resulting in infertility. Researchers use ODF1 antibody to study spermatogenesis, sperm structure, and male fertility.

Clinically, ODF1 mutations or dysregulation have been associated with asthenozoospermia and male infertility. Studies in mice show that ODF1 knockout results in male sterility due to defective sperm tails. Because sperm tail defects are a common cause of infertility, ODF1 is a biomarker of reproductive health. NSJ Bioreagents provides ODF1 antibody for fertility and reproductive biology research.

Experimentally, ODF1 antibody is used in western blotting to detect the ~27 kDa protein, in immunofluorescence to visualize sperm tail localization, and in immunohistochemistry of testicular sections. Co-immunoprecipitation with ODF1 antibody reveals interactions with cytoskeletal partners critical for flagellar integrity.

UniProt: Q14990