GK2 Antikörper (N-Term)

Produktnummer ABIN7879604

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch GK2 in WB, IF, ICC und FACS. Es zeigt Reaktivität gegenüber Proben von Human, Ratte und Maus.

Kurzübersicht für GK2 Antikörper (N-Term) (ABIN7879604)

Target: GK2 (Glycerol Kinase 2 (GK2))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-GK2 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: GK2 Antibody / Glycerol kinase 2

Aufreinigung: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human GK2 was used as the immunogen for the GK2 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the GK2 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the GK2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu GK2

Target: GK2 (Glycerol Kinase 2 (GK2))

Andere Bezeichnung: GK2

Hintergrund: GK2 antibody detects Glycerol kinase 2, encoded by the GK2 gene on chromosome 4q13.3. GK2 antibody is commonly applied in studies of glycerol metabolism, mitochondrial energy regulation, and sperm function. GK2 belongs to the glycerol kinase family, enzymes that phosphorylate glycerol to glycerol-3-phosphate, a critical intermediate in lipid metabolism and glycolysis. Unlike the ubiquitously expressed GK1, GK2 is primarily expressed in testis and sperm, where it plays a role in fertility and energy production. It localizes to mitochondria, highlighting its role in coupling glycerol metabolism with energy pathways.

Structurally, GK2 is a ~61 kDa enzyme composed of conserved ATP-binding and substrate-binding domains typical of kinases in this family. It lacks some regulatory motifs present in GK1, leading to distinct enzymatic properties and tissue specificity. The mitochondrial localization sequence targets GK2 to the inner membrane, enabling direct integration into oxidative phosphorylation networks.

Functionally, GK2 catalyzes the phosphorylation of glycerol to glycerol-3-phosphate, fueling glycolysis and triglyceride synthesis. In sperm, this activity supports energy metabolism necessary for motility and fertilization. GK2 also contributes to mitochondrial glycerol-3-phosphate shuttle activity, linking cytosolic NADH reoxidation with the respiratory chain. Knockdown or disruption of GK2 leads to impaired sperm motility and male infertility, emphasizing its critical reproductive role. Researchers use GK2 antibody to study sperm metabolism, mitochondrial function, and lipid biochemistry.

Clinically, GK2 mutations or dysfunction have been associated with infertility and metabolic syndromes involving lipid dysregulation. Because GK2 expression is highly specific to testis, it has been investigated as a biomarker of male fertility potential. GK2 is also implicated in mitochondrial biology disorders, where energy coupling is impaired. Its restricted expression compared to GK1 makes it an attractive target for reproductive health research. NSJ Bioreagents provides GK2 antibody as a validated tool for mitochondrial and fertility research.

Experimentally, GK2 antibody is applied in western blotting to detect the ~61 kDa protein, in immunofluorescence microscopy to localize it in mitochondria, and in immunohistochemistry to examine expression in testis tissue. Enzyme assays combined with GK2 antibody detection enable correlation of enzymatic activity with localization and expression.

UniProt: Q14410