PAX7 Antikörper (Middle Region)

Produktnummer ABIN7879536

Der Kaninchen Polyklonal anti-PAX7 Antikörper wird verwendet zum Nachweis von PAX7 in Proben von Human und Maus. Er wurde validiert für WB und FACS.

Kurzübersicht für PAX7 Antikörper (Middle Region) (ABIN7879536)

Target: PAX7 (Paired Box 7 (PAX7))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PAX7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Produktdetails anti-PAX7 Antikörper

Bindungsspezifität: Middle Region

Verwendungszweck: PAX7 Antibody / Paired box protein 7

Aufreinigung: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PAX7 was used as the immunogen for the PAX7 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the PAX7 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the PAX7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu PAX7

Target: PAX7 (Paired Box 7 (PAX7))

Andere Bezeichnung: PAX7

Hintergrund: PAX7 antibody detects Paired box protein 7, a transcription factor critical for myogenesis, neural crest formation, and satellite cell maintenance. PAX7 belongs to the paired box family of transcriptional regulators that control early developmental programs through DNA binding and transcriptional activation. The PAX7 antibody is widely used in developmental and muscle biology to study stem cell regulation and tissue regeneration.

PAX7 is encoded by the PAX7 gene located on human chromosome 1p36.13. The protein contains a paired DNA-binding domain, a homeodomain, and a C-terminal transactivation domain. During embryonic development, PAX7 is expressed in neural crest cells and the myogenic progenitor population of the somites. In adults, it marks quiescent muscle satellite cells responsible for postnatal muscle repair and regeneration.

The PAX7 antibody identifies a 55 kilodalton nuclear protein by western blot and demonstrates strong nuclear staining in satellite cells and developing muscle fibers. Loss of PAX7 disrupts myogenic lineage specification, resulting in impaired muscle growth. Functionally, PAX7 activates myogenic regulatory factors such as MYOD and MYF5 and promotes self-renewal of satellite cells through repression of differentiation pathways.

Beyond muscle tissue, PAX7 contributes to neurogenesis, pituitary development, and craniofacial patterning. Dysregulation of PAX7 is linked to rhabdomyosarcoma, where gene fusions with FOXO1 create oncogenic transcriptional drivers. NSJ Bioreagents provides a validated PAX7 antibody, enabling precise detection of muscle progenitors and developmental regulators.

UniProt: P23759