RAB27B Antikörper (C-Term)

Produktnummer ABIN7877467

Der Kaninchen Polyklonal anti-RAB27B Antikörper (ABIN7877467) detektiert spezifisch RAB27B in WB, IHC, FACS, IF und ICC. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für RAB27B Antikörper (C-Term) (ABIN7877467)

Target: RAB27B (RAB27B, Member RAS Oncogene Family (RAB27B))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAB27B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-RAB27B Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: RAB27B Antibody / Ras-related protein Rab-27B

Aufreinigung: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human RAB27B was used as the immunogen for the RAB27B antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the RAB27B antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the RAB27B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu RAB27B

Target: RAB27B (RAB27B, Member RAS Oncogene Family (RAB27B))

Andere Bezeichnung: RAB27B

Hintergrund: RAB27B antibody detects Ras-related protein Rab-27B, a member of the Rab family of small GTPases that regulates vesicle docking, secretion, and exocytosis. RAB27B plays key roles in platelet dense granule release, melanosome transport, and hormone secretion. The RAB27B antibody is widely used in cell biology, endocrinology, and neurobiology to study regulated exocytosis and vesicle trafficking.

RAB27B is encoded by the RAB27B gene located on human chromosome 18q21.32. The protein is approximately 221 amino acids long and cycles between active GTP-bound and inactive GDP-bound states. RAB27B localizes to secretory vesicles and interacts with effector proteins such as SYTL2, MLPH, and Slac2 family members, facilitating vesicle tethering to the plasma membrane before exocytosis.

The RAB27B antibody detects a 25 kilodalton protein by western blot and shows vesicular punctate staining under confocal microscopy. RAB27B functions in a wide range of cell types, including platelets, endocrine cells, and neurons, where it regulates secretion of serotonin, insulin, and neurotransmitters. In melanocytes, RAB27B cooperates with RAB27A to control melanosome transport and pigment distribution.

Dysregulation of RAB27B has been linked to cancer metastasis, neurodegenerative disease, and immune dysfunction. Overexpression enhances invasive potential in breast and colorectal cancers by promoting secretion of exosomes that modify the tumor microenvironment. Mutations or reduced function lead to granule secretion defects and pigmentation abnormalities.

Because RAB27B regulates exocytosis and vesicle transport, it serves as a model for understanding intracellular trafficking mechanisms and communication between cells. NSJ Bioreagents provides a validated RAB27B antibody optimized for western blot, immunofluorescence, and vesicle transport assays, supporting studies in secretion, membrane dynamics, and cellular signaling.

UniProt: O00194