PCGF6 Antikörper (C-Term)

Produktnummer ABIN7877457

Der Kaninchen Polyklonal anti-PCGF6 Antikörper wird verwendet zum Nachweis von PCGF6 in Proben von Human, Maus und Ratte. Er wurde validiert für WB und FACS.

Kurzübersicht für PCGF6 Antikörper (C-Term) (ABIN7877457)

Target: PCGF6 (Polycomb Group Ring Finger 6 (PCGF6))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PCGF6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Produktdetails anti-PCGF6 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: PCGF6 Antibody / Polycomb group RING finger protein 6

Aufreinigung: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PCGF6 was used as the immunogen for the PCGF6 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the PCGF6 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the PCGF6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu PCGF6

Target: PCGF6 (Polycomb Group Ring Finger 6 (PCGF6))

Andere Bezeichnung: PCGF6

Hintergrund: PCGF6 antibody detects Polycomb group RING finger protein 6, a transcriptional repressor that functions as part of noncanonical Polycomb repressive complex 1.6 (PRC1.6). Encoded by the PCGF6 gene on chromosome 10p15.3, this protein belongs to the Polycomb group (PcG) family, which maintains transcriptional repression of developmental and cell cycle genes through histone modification and chromatin compaction. PCGF6 acts as a key scaffold within PRC1.6, recruiting additional subunits including L3MBTL2, RING1A/B, and E2F6, to mediate histone H2A monoubiquitination and gene silencing independent of canonical PRC1 complexes.

PCGF6 contains a RING finger domain responsible for interacting with the ubiquitin ligases RING1A/B, promoting histone modification that represses gene transcription. Through association with the transcriptional repressors E2F6 and MGA, PCGF6 regulates genes involved in cell proliferation, germline maintenance, and differentiation. It is expressed in embryonic stem cells, germ cells, and certain somatic tissues, where it contributes to pluripotency and developmental gene silencing. PCGF6 activity ensures appropriate repression of lineage-specific genes during embryogenesis and germ cell development.

The PCGF6 antibody is used in epigenetics, developmental biology, and transcriptional regulation studies to investigate Polycomb complex function and chromatin remodeling. Western blot analysis detects a 37 kilodalton band corresponding to PCGF6, and immunofluorescence reveals nuclear punctate staining associated with chromatin regions. This antibody enables researchers to track PRC1.6 distribution and assess its role in transcriptional repression and stem cell identity.

Aberrant expression of PCGF6 has been implicated in cancer and developmental disorders. Overexpression supports cell proliferation and transformation, while depletion can impair germ cell specification and induce differentiation. By regulating histone ubiquitination and chromatin architecture, PCGF6 acts as a molecular link between epigenetic control and transcriptional silencing. The PCGF6 antibody provides a dependable tool for studying these mechanisms in both stem cell and cancer research. NSJ Bioreagents provides this antibody validated for its applications, ensuring consistent detection of Polycomb-associated pathways.

UniProt: Q9BYE7