PASK Antikörper (C-Term)

Produktnummer ABIN7877453

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch PASK in FACS. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für PASK Antikörper (C-Term) (ABIN7877453)

Target: PASK (PAS Domain Containing Serine/threonine Kinase (PASK))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PASK Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS)

Produktdetails anti-PASK Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: PASK Antibody / PAS kinase

Aufreinigung: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PASK was used as the immunogen for the PASK antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the PASK antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the PASK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu PASK

Target: PASK (PAS Domain Containing Serine/threonine Kinase (PASK))

Andere Bezeichnung: PASK

Hintergrund: PASK antibody detects PAS domain-containing serine/threonine-protein kinase, an evolutionarily conserved enzyme that functions as a nutrient and energy sensor linking cellular metabolism with transcriptional control. Encoded by the PASK gene on chromosome 2q37.3, this kinase contains a C-terminal catalytic domain and N-terminal PAS (Per-Arnt-Sim) domains that sense changes in oxygen, redox potential, and small-molecule metabolites. PASK acts as a molecular integrator that coordinates energy homeostasis, glucose metabolism, and mitochondrial biogenesis in response to nutrient availability. The PAS domains enable regulation of its kinase activity through allosteric mechanisms, allowing PASK to respond dynamically to metabolic states.

PASK is broadly expressed in liver, skeletal muscle, pancreatic islets, and the brain, tissues that exhibit strong metabolic plasticity. In hepatocytes, PASK influences glycogen synthesis and gluconeogenic pathways, while in pancreatic beta cells it modulates insulin secretion. Mice lacking PASK exhibit altered glucose tolerance, reduced hepatic glycogen storage, and impaired mitochondrial function, demonstrating its role in metabolic adaptation. PASK phosphorylates transcriptional coactivators such as PGC-1alpha and CREB-regulated transcriptional coactivator 2 (CRTC2), linking nutrient signaling with gene expression that governs oxidative metabolism.

The PASK antibody is used in metabolic research to detect PASK expression and activation across tissues. Western blotting typically identifies a ~160 kilodalton band corresponding to the full-length kinase, while immunofluorescence localizes it to the cytoplasm and nucleus depending on cell type. In mammalian systems, PASK activity rises during fasting and decreases in nutrient-replete conditions, reflecting its regulatory role in energy balance. Because dysregulation of PASK signaling has been associated with diabetes, obesity, and mitochondrial dysfunction, this antibody provides an important tool for investigating metabolic signaling networks.

Recent studies also implicate PASK in circadian rhythm regulation and differentiation of muscle and stem cells. Its PAS domains act as environmental sensors, adjusting transcriptional programs to oxygen and redox changes. PASK interacts with AMPK and mTOR pathways, highlighting its integration into major metabolic signaling circuits. The PASK antibody enables researchers to explore these regulatory interactions and to characterize PASK as a potential therapeutic target in metabolic disease and aging. NSJ Bioreagents provides this antibody validated for its applications, ensuring precise detection across human and model systems.

UniProt: Q96RG2

Pathways: Regulation of Carbohydrate Metabolic Process