SNX27 Antikörper (AA 96-541)

Produktnummer ABIN7877254

Der Kaninchen Polyklonal anti-SNX27 Antikörper (ABIN7877254) detektiert spezifisch SNX27 in ELISA, WB und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für SNX27 Antikörper (AA 96-541) (ABIN7877254)

Target: SNX27 (Sorting Nexin 27 (SNX27))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SNX27 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Produktdetails anti-SNX27 Antikörper

Bindungsspezifität: AA 96-541

Verwendungszweck: SNX27 Antibody / Sorting nexin-27

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human SNX27 recombinant protein (Position: R96-T541) was used as the immunogen for the SNX27 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the SNX27 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the SNX27 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu SNX27

Target: SNX27 (Sorting Nexin 27 (SNX27))

Andere Bezeichnung: SNX27

Hintergrund: SNX27 antibody detects Sorting nexin-27, encoded by the SNX27 gene on chromosome 1q21.1. SNX27 antibody is widely used in studies of endosomal trafficking, receptor recycling, and neurological disorders. Sorting nexins are a diverse family of endosomal proteins characterized by PX domains that bind phosphoinositides. SNX27 is unique among sorting nexins in containing a PDZ domain, which recognizes PDZ-binding motifs in target proteins, and an FERM-like domain, which regulates interactions with cytoskeletal and signaling proteins. This structural versatility enables SNX27 to link endosomal sorting with cell signaling and polarity.

Structurally, SNX27 is a ~61 kDa protein containing three functional domains: an N-terminal PDZ domain, a central PX domain, and a C-terminal FERM-like domain. The PX domain mediates binding to phosphatidylinositol-3-phosphate in endosomal membranes, targeting SNX27 to recycling endosomes. The PDZ domain recognizes a wide variety of membrane proteins with C-terminal PDZ-binding motifs, including receptors, transporters, and channels.

Functionally, SNX27 promotes endosome-to-plasma membrane recycling of nutrient transporters, GPCRs, and signaling receptors. This ensures surface availability of proteins like glucose transporters (GLUT1), beta-adrenergic receptors, and NMDA receptors, which are essential for metabolism, neurotransmission, and signaling. SNX27 works within the retromer complex to mediate cargo selection and retrieval. Loss of SNX27 impairs receptor recycling, leading to reduced nutrient uptake and signaling defects. Researchers employ SNX27 antibody to study receptor trafficking, endosome biology, and neurological disease mechanisms.

Clinically, SNX27 dysfunction is associated with Down syndrome, neurodevelopmental delay, and epilepsy. Reduced SNX27 expression contributes to synaptic dysfunction and impaired glutamate receptor recycling in Down syndrome models. Its broad role in trafficking links it to metabolic disease, immunity, and cancer, where altered receptor localization influences signaling outcomes. NSJ Bioreagents provides SNX27 antibody to support neuroscience, immunology, and cell biology research.

Experimentally, SNX27 antibody is applied in western blotting to detect the ~61 kDa protein, in immunofluorescence microscopy to study endosomal distribution, and in immunohistochemistry to examine tissue-specific patterns. Co-immunoprecipitation with SNX27 antibody identifies PDZ-binding cargo proteins and retromer complex partners.

UniProt: Q96L92