PRPF4B Antikörper (AA 94-1007)

Produktnummer ABIN7877188

Der Kaninchen Polyklonal anti-PRPF4B Antikörper (ABIN7877188) detektiert spezifisch PRPF4B in WB, ELISA, IF, FACS und ICC. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für PRPF4B Antikörper (AA 94-1007) (ABIN7877188)

Target: PRPF4B (Serine/threonine-Protein Kinase PRP4 Homolog (PRPF4B))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRPF4B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-PRPF4B Antikörper

Bindungsspezifität: AA 94-1007

Verwendungszweck: PRP4K Antibody / Pre-mRNA-processing factor 4B kinase

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human PRP4K recombinant protein (Position: P94-I1007) was used as the immunogen for the PRP4K antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the PRP4K antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the PRP4K antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu PRPF4B

Target: PRPF4B (Serine/threonine-Protein Kinase PRP4 Homolog (PRPF4B))

Andere Bezeichnung: PRP4K

Hintergrund: PRP4K antibody detects Pre-mRNA-processing factor 4B kinase, also known as PRP4 kinase (PRPF4B), a dual-specificity serine/threonine-tyrosine kinase involved in pre-mRNA splicing and signal transduction. The UniProt recommended name is Serine/threonine-protein kinase PRP4 homolog (PRPF4B), and alternate names include pre-mRNA-processing factor 4 homolog B, PRP4 kinase, and PRPF4-related kinase. PRP4K functions at the intersection of RNA processing and cell signaling, linking splicing machinery dynamics to stress and growth pathways.

PRP4K antibody targets a nuclear kinase that associates with spliceosomal components and regulates phosphorylation of splicing factors such as SF3B1 and PRP6, influencing spliceosome assembly and mRNA maturation. PRP4K activity is essential for accurate exon recognition and alternative splicing decisions, affecting transcriptome diversity. The protein also modulates mitotic checkpoint signaling through interaction with BUB1 and MAD1 complexes, ensuring proper chromosome segregation. Dysregulation of PRPF4B expression has been associated with aberrant splicing events, genomic instability, and tumorigenesis.

The PRPF4B gene is located on chromosome 6p21.31 and encodes a protein of approximately 1007 amino acids containing an N-terminal kinase domain and a C-terminal proline-rich regulatory region. PRP4K phosphorylates SR-rich splicing proteins, coordinating RNA processing with transcriptional elongation and stress responses. Its activity is regulated by phosphorylation, autoinhibition, and interactions with 14-3-3 proteins. Elevated expression of PRP4K has been observed in cancers such as breast, ovarian, and glioblastoma, where it influences epithelial-mesenchymal transition (EMT) and drug resistance phenotypes.

Researchers employ PRP4K antibody for western blotting, immunofluorescence, and immunoprecipitation to monitor nuclear localization, post-translational modifications, and spliceosomal associations. The kinase also participates in response to genotoxic stress and growth factor signaling via ERK and JNK cascades. Functional depletion studies reveal that loss of PRP4K impairs cell cycle progression and enhances sensitivity to DNA-damaging agents. Because PRP4K integrates splicing control with cell survival signaling, it has emerged as a potential therapeutic target for cancer intervention. NSJ Bioreagents provides validated reagents for detecting human, mouse, and rat PRP4K suitable for cellular, molecular, and proteomic analyses.

UniProt: Q13523