NPRL2 Antikörper (AA 90-380)

Produktnummer ABIN7877107

Der Kaninchen Polyklonal anti-NPRL2 Antikörper (ABIN7877107) detektiert spezifisch NPRL2 in WB, ELISA und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Maus und Ratte.

Kurzübersicht für NPRL2 Antikörper (AA 90-380) (ABIN7877107)

Target: NPRL2 (Nitrogen Permease Regulator-Like 2 (S. Cerevisiae) (NPRL2))

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NPRL2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-NPRL2 Antikörper

Bindungsspezifität: AA 90-380

Verwendungszweck: NPRL2 Antibody / Nitrogen permease regulator-like 2

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human NPRL2 recombinant protein (Position: D90-K380) was used as the immunogen for the NPRL2 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the NPRL2 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the NPRL2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu NPRL2

Target: NPRL2 (Nitrogen Permease Regulator-Like 2 (S. Cerevisiae) (NPRL2))

Andere Bezeichnung: NPRL2

Hintergrund: NPRL2 antibody detects Nitrogen permease regulator-like 2, a component of the GATOR1 complex that inhibits mTORC1 signaling in response to amino acid deprivation. NPRL2 functions as a tumor suppressor and metabolic checkpoint regulator, linking nutrient sensing to cell growth and autophagy. The NPRL2 antibody is widely used in metabolism, oncology, and signal transduction research to study mTOR regulation, nutrient signaling, and tumor suppression.

NPRL2 is encoded by the NPRL2 gene located on human chromosome 3p21.3, a region frequently deleted in several cancers. The protein is approximately 380 amino acids long and forms a complex with NPRL3 and DEPDC5 to constitute the GATOR1 inhibitory module. NPRL2 localizes to both cytoplasm and lysosomes, where it acts upstream of Rag GTPases to suppress mTORC1 activity under nutrient-limiting conditions.

The NPRL2 antibody detects a 45 kilodalton band by western blot and reveals punctate cytoplasmic staining consistent with lysosomal localization. NPRL2 is required for proper cellular adaptation to nutrient stress, promoting autophagy and metabolic reprogramming. Loss of NPRL2 results in constitutive mTORC1 activation, uncontrolled cell proliferation, and impaired stress response.

As a tumor suppressor, NPRL2 modulates DNA damage repair and enhances sensitivity to chemotherapeutic agents such as cisplatin. Reduced NPRL2 expression has been documented in lung, colorectal, and breast cancers, correlating with poor prognosis and therapy resistance. Restoring NPRL2 expression reactivates nutrient stress signaling and suppresses tumor growth through mTOR inhibition and apoptosis induction.

Through its dual role in nutrient sensing and cancer suppression, NPRL2 serves as a key regulator of cellular homeostasis. NSJ Bioreagents provides a validated NPRL2 antibody optimized for its applications, supporting research into mTOR signaling, autophagy regulation, and tumor suppression mechanisms.

UniProt: Q8WTW4