SHMT2 Antikörper (AA 9-504)

Produktnummer ABIN7877091

Der Kaninchen Polyklonal anti-SHMT2 Antikörper wird verwendet zum Nachweis von SHMT2 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC, IF, ELISA, IP, ICC und FACS.

Kurzübersicht für SHMT2 Antikörper (AA 9-504) (ABIN7877091)

Target: SHMT2 (serine Hydroxymethyltransferase 2 (Mitochondrial) (SHMT2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SHMT2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-SHMT2 Antikörper

Bindungsspezifität: AA 9-504

Verwendungszweck: SHMT2 Antibody / Serine hydroxymethyltransferase

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human SHMT2 recombinant protein (Position: A9-H504) was used as the immunogen for the SHMT2 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the SHMT2 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the SHMT2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu SHMT2

Target: SHMT2 (serine Hydroxymethyltransferase 2 (Mitochondrial) (SHMT2))

Andere Bezeichnung: SHMT2

Hintergrund: The SHMT2 antibody targets Serine hydroxymethyltransferase, mitochondrial, an enzyme encoded by the SHMT2 gene that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylenetetrahydrofolate. This reaction is central to one-carbon metabolism, providing key intermediates for nucleotide synthesis, methylation reactions, and redox regulation. Serine hydroxymethyltransferase, mitochondrial operates within the mitochondrial folate cycle and contributes to biosynthetic and energetic demands in proliferating cells. The SHMT2 antibody provides a reliable reagent for studying one-carbon metabolism, mitochondrial function, and cancer metabolism.

Serine hydroxymethyltransferase, mitochondrial forms homotetramers that utilize pyridoxal phosphate (PLP) as a cofactor for catalysis. Its activity links amino acid metabolism with folate-mediated one-carbon flux, coordinating mitochondrial and cytosolic metabolic networks. The SHMT2 antibody enables visualization of this enzyme within mitochondria, supporting studies of its spatial regulation and contribution to biosynthetic pathways under nutrient limitation or hypoxic conditions. SHMT2 expression increases in rapidly dividing cells to sustain nucleotide production and redox balance.

Aberrant activation of one-carbon metabolism, including SHMT2 upregulation, is a hallmark of cancer metabolism. Overexpression of Serine hydroxymethyltransferase, mitochondrial enhances proliferation and resistance to oxidative stress by supplying reduced folate cofactors and NADPH. The SHMT2 antibody facilitates detection of this metabolic enzyme in tumor tissues and cell lines, aiding evaluation of its role in tumorigenesis and metabolic adaptation. SHMT2 has been proposed as a potential therapeutic target, as inhibition disrupts nucleotide synthesis and redox homeostasis in cancer cells.

Beyond oncology, Serine hydroxymethyltransferase, mitochondrial contributes to neural development, mitochondrial respiration, and cellular methylation capacity. It provides one-carbon units necessary for formate generation and DNA methylation reactions that influence epigenetic regulation. The SHMT2 antibody supports research into these metabolic control points, enabling functional analysis of folate cycle dynamics in metabolic disorders, neurodegenerative diseases, and aging.

The SHMT2 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing clear mitochondrial staining patterns. NSJ Bioreagents provides this antibody with validated specificity for consistent performance in biochemistry, metabolism, and cancer biology research. By enabling detailed study of Serine hydroxymethyltransferase, mitochondrial expression and activity, the SHMT2 antibody supports discovery into one-carbon metabolism and mitochondrial regulation under physiological and pathological conditions.

UniProt: P34897