NPAT Antikörper (AA 87-1191)

Produktnummer ABIN7877013

Der Kaninchen Polyklonal anti-NPAT Antikörper wird verwendet zum Nachweis von NPAT in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IF, IHC, ELISA, FACS und ICC.

Kurzübersicht für NPAT Antikörper (AA 87-1191) (ABIN7877013)

Target: NPAT (Nuclear Protein, Ataxia-Telangiectasia Locus (NPAT))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NPAT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-NPAT Antikörper

Bindungsspezifität: AA 87-1191

Verwendungszweck: NPAT Antibody / Nuclear protein of the ATM locus

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human NPAT recombinant protein (Position: K87-Q1191) was used as the immunogen for the NPAT antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the NPAT antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the NPAT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu NPAT

Target: NPAT (Nuclear Protein, Ataxia-Telangiectasia Locus (NPAT))

Andere Bezeichnung: NPAT

Hintergrund: NPAT antibody detects Nuclear protein, ataxia-telangiectasia locus, encoded by the NPAT gene on chromosome 11q22.3. NPAT antibody is used to study this nuclear regulatory protein, which coordinates cell cycle progression, histone gene transcription, and DNA damage responses. NPAT was identified as a substrate of ATM kinase, linking it to DNA damage signaling and ataxia-telangiectasia biology. It is ubiquitously expressed, with high levels in proliferating cells, particularly in tissues with active cell division such as bone marrow and lymphoid organs.

Structurally, NPAT contains multiple phosphorylation sites targeted by cyclin-dependent kinases (CDKs) and ATM. These modifications regulate its activity during cell cycle transitions and in response to DNA damage. NPAT localizes to Cajal bodies and histone gene clusters, where it acts as a transcriptional co-activator for histone genes. This localization is mediated by its interaction with the transcription factor HiNF-P, forming the HiNF-P/NPAT complex essential for histone gene transcription.

Functionally, NPAT is indispensable for S-phase progression. It activates histone gene transcription at the G1/S transition, ensuring histone supply for DNA replication. NPAT also influences DNA repair by participating in ATM signaling, linking DNA damage sensing to transcriptional regulation. Knockout studies in mice show embryonic lethality, confirming NPAT's essential role in development. Researchers apply NPAT antibody to study its functions in chromatin biology, cell cycle regulation, and DNA repair pathways.

Clinically, NPAT is significant in cancer biology. Overexpression is observed in certain malignancies, where it supports uncontrolled proliferation. Altered NPAT activity may contribute to genomic instability and defective DNA damage responses. Its regulation by ATM connects NPAT to ataxia-telangiectasia, a neurodegenerative and cancer-prone disorder caused by ATM mutations. NPAT is also a potential biomarker of proliferation, with expression correlating to cell cycle activity. NSJ Bioreagents offers NPAT antibody to facilitate studies in DNA replication, chromatin remodeling, and cancer research.

Experimentally, NPAT antibody is used in western blotting to detect the highly phosphorylated 230-260 kDa protein, in immunofluorescence to visualize its localization to histone gene clusters, and in immunohistochemistry to assess proliferation in tissue samples. Immunoprecipitation with NPAT antibody helps characterize its interactions with HiNF-P and ATM kinase. These tools enable detailed exploration of its cell cycle and DNA repair functions.

UniProt: Q14207