RPAP3 Antikörper (AA 84-604)

Produktnummer ABIN7876961

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch RPAP3 in WB und ELISA. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für RPAP3 Antikörper (AA 84-604) (ABIN7876961)

Target: RPAP3 (RNA Polymerase II Associated Protein 3 (RPAP3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RPAP3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-RPAP3 Antikörper

Bindungsspezifität: AA 84-604

Verwendungszweck: RPAP3 Antibody / RNA polymerase II-associated protein 3

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human RPAP3 recombinant protein (Position: R84-E604) was used as the immunogen for the RPAP3 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the RPAP3 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the RPAP3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu RPAP3

Target: RPAP3 (RNA Polymerase II Associated Protein 3 (RPAP3))

Andere Bezeichnung: RPAP3

Hintergrund: RPAP3 antibody detects RNA polymerase II-associated protein 3, encoded by the RPAP3 gene on chromosome 12q24.31. RPAP3 antibody is commonly used in transcription, chaperone, and cancer research. RPAP3 is a component of the R2TP cochaperone complex, which cooperates with HSP90 and HSP70 to stabilize and assemble large macromolecular complexes. It is involved in the maturation of RNA polymerase II, snoRNPs, PIKKs, and other critical protein assemblies.

Structurally, RPAP3 is a ~75 kDa protein with multiple tetratricopeptide repeat (TPR) domains, which mediate protein-protein interactions. These domains allow RPAP3 to bridge HSP90 and PIH1D1, central elements of the R2TP complex. Its N-terminal domain anchors it to the cochaperone machinery, while its C-terminal region facilitates substrate recognition and client protein assembly.

Functionally, RPAP3 stabilizes RNA polymerase II subunits, enabling proper transcriptional initiation and elongation. It also regulates assembly of snoRNPs, which support ribosomal RNA modification, and PIKKs, which are central to DNA damage response, nutrient sensing, and stress signaling. Researchers use RPAP3 antibody to explore protein complex maturation, transcriptional regulation, and chaperone pathways.

Clinically, RPAP3 dysregulation has been linked to cancer progression. Because it stabilizes oncogenic PIKKs such as mTOR, ATR, and ATM, RPAP3 supports tumor growth and therapy resistance. Its expression patterns may serve as biomarkers for prognosis and treatment response. NSJ Bioreagents provides RPAP3 antibody to support transcription, DNA damage response, and cancer research.

Experimentally, RPAP3 antibody is applied in western blotting to detect the ~75 kDa protein, in immunoprecipitation to study R2TP complex partners, and in immunofluorescence microscopy to visualize nuclear and cytoplasmic distribution. Chromatin immunoprecipitation using RPAP3 antibody helps examine transcriptional regulation via RNA polymerase II stabilization.

UniProt: Q9H6T3